[Genetics] 50 cm capillaries

[Tanja Pyhäjärvi]

Hi all sequencer-users!

I just wanted to remind everybody about the possibility to use also 
longer 50 cm capillaries instead of 35 cm ones. With long run-time and 
long capillaries, it is possible to get about 900 bp good sequence. You 
just need to specify that in sample sheet and put it to the correct 
folder in ABI-server, otherwise the procedure is the same for both 
capillaries. Run takes longer time with long capillaries but on the 
other hand you don't need to do as many sequencing reactions or use so 
many different primers for sequencing longer srtetches compared to 
shorter capillaries. At least in our group using the longer capillaries 
has really resulted longer sequences. In some cases you might need to 
use more BDT-mix (1/4 reaction has been sufficient for us).

So far the policy has been that capillaries have been changed when there 
has been several plates for longer capillaries (there is no point 
changing the capillaries for each plate), not more often than once a week.

Hannele is on holiday 23.12.-2.1. and there ABI won't be running during 
that time.

Regards
Tanja