Bco-kieppi March 2012

bco-kieppi@lists.oulu.fi

5 participants
16 discussions

Stratagene qPCR computer changed
by Antti Viklund 30 Mar '12

30 Mar '12

Hi all, The computer for the qPCR was changed. Hopefully it will work normally again. BRs, Antti -- ******************************************** Antti Viklund, Project Engineer University of Oulu, Biocenter Oulu P.O. Box 5000 (Aapistie 5 A) FIN-90014 UNIVERSITY OF OULU, Finland Tel. + 358-8-537 6124 (Mobile 040-5303941) e-mail: antti.viklund(a)oulu.fi BIOCENTER URL: http://www.biocenter.oulu.fi (internal mail: 5 BIOCENTER)

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Strategene QPCR computer is broken
by Antti Viklund 29 Mar '12

29 Mar '12

Hi all, I have to change the computer that controls the Stratagene QPCR device in Kieppi 4th floor. I don't know yet how long it will take but I will try to get it ready tomorrow. Best regards, Antti -- ******************************************** Antti Viklund, Project Engineer University of Oulu, Biocenter Oulu P.O. Box 5000 (Aapistie 5 A) FIN-90014 UNIVERSITY OF OULU, Finland Tel. + 358-8-537 6124 (Mobile 040-5303941) e-mail: antti.viklund(a)oulu.fi BIOCENTER URL: http://www.biocenter.oulu.fi (internal mail: 5 BIOCENTER)

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TODAY the First Spring 2012 Seminar of Inst. Biomed. by Dr. Sohvi Hörkkö - Next Thursday 22.3. klo. 15.15
by Seppo Vainio 22 Mar '12

22 Mar '12

Subject: Invitation to the First Spring 2012 Seminar of Inst. Biomed. by Dr. Sohvi Hörkkö - Next Thursday 22.3. klo. 15.15 Dear All, It is my pleasure to advertise the first soring 2012 seminar of Institute of Biomedicine. The lecture will be held in P117, close to the Dept. of Parthology. The talk will be given by Dr. Sohvi Hörkkö. Here are the deatails: Place: P117 Time: 15.15-16.00 Speaker: Dr. Sohvi Hörkkö: "The role of humoral immune system in atherogenesis" See you all there - to get exposed to issues of immunity to hear advancements in this major health problem . Yours, Seppo Seppo Vainio, prof. Dev. Biol. Vice Director of Inst. Biomed. Science Issues

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Coffee and cake on Wednesday!
by Pirkko Huhtala 22 Mar '12

22 Mar '12

Dear BCO Kieppi People, BCO coordinator Sinikka Eskelinen has got a new job in the central administration and Pirkko Huhtala has been appointed as a new coordinator. Sinikka still continues in Kieppi as a light microscopy coordinator and has moved to the 3rd floor whereas Pirkko is sitting in the 4th floor coordinator's office. We kindly invite you to celebrate with us the "Changing rooms" event with coffee, cake and something sparkling. Time: Wednesday, March 28th 12.30- Place: BCO 4th floor meeting room 487B Welcome! Best regards, Sinikka Eskelinen, Former BCO Coordinator and Pirkko Huhtala, Current BCO Coordinator ****************************** Sinikka Eskelinen Director, Docent, Ph.D. Research Services mail address: P.O. Box 8000, FI-90014 University of Oulu, Finland street address: Pentti Kaiterankatu 1, Linnanmaa Campus, University of Oulu, Finland Tel: +358-40-1800855 e-mail: sinikka.eskelinen(a)oulu.fi<mailto:sinikka.eskelinen@oulu.fi> ******************************************** Pirkko Huhtala, Ph.D. Coordinator Biocenter Oulu University of Oulu Mailing address: P.O.Box 5000, FIN-90014 University of Oulu Finland Street address: Aapistie 5A, FIN-90220 Oulu Tel. +358-8-537 6101, +358-40-189 7467 e-mail pirkko.huhtala(a)oulu.fi www.oulu.fi/biocenter<http://www.oulu.fi/biocenter>

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Fwd: Re: [Lbiok] GelDoc instrument
by Antti Viklund 21 Mar '12

21 Mar '12

-------- Original Message -------- Subject: Re: [Lbiok] GelDoc instrument Date: Tue, 20 Mar 2012 14:50:58 +0000 From: Hongmin Tu <Hongmin.Tu(a)oulu.fi> To: lbiok(a)lists.oulu.fi <lbiok(a)lists.oulu.fi> Hi all the gel imaging workers, The new Bio-Rad GelDoc instrument arrived at 10 am today, and it was installed immediately. Some of us have learned and tested the system. So others can ask us for the help if you need. I will soon test the green dye Midori with the “green” filter. If it is working, we shall stop using EtBr as soon as possible. There is a Blue Conversion Safe Screen + Googles for protecting ourselves and the DNA samples from the UV light. Please consider it when you cut the gel bands. The green filter and the Blue Screen have also been installed to BCO “old” GelDoc. The new software Image Lab has no limitation of the license, so it can be installed to any computer. The computer administrators will help us to do it. Unfortunately the BCO old instrument cannot be controlled by this new software, but the data in the saved file can be analyzed by the new version. We have thermal printers for both instruments. I would like to mention the risk of the thermal printer paper. Many of us were listening to the BCO seminar last week with the topic of “Breast cancer and Plastic”. Cathrin Brisken mentioned the cancer-risk chemical BPA, which is also the material for the thermal paper. If you want to know more about this, please refer to this article in English: http://www.sciencenews.org/view/generic/id/61740/title/Cashiers_may_face_sp… Anyway, we should wash our hands more carefully after touching the thermal paper. A “Quick Start” instruction on using the instrument will be ready soon. BWs, Hongmin Hongmin Tu, Ph.D. Biocenter and Department of Medical Biochemistry and Molecular Biology University of Oulu Aapistie 7 P.O.Box 5000 90014 Oulu Finland Tel: +358-8-5375821, +358-(0)41-4365643 Fax:+358-8-5375811 email: hongmin.tu(a)oulu.fi

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Invitation to the First Spring 2012 Seminar of Inst. Biomed. by Dr. Sohvi Hörkkö - Next Thursday 22.3. klo. 15.15
by Seppo Vainio 20 Mar '12

20 Mar '12

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Fwd: [Lbiok] New GelDoc instrument
by Antti Viklund 19 Mar '12

19 Mar '12

There will be also some updates for the older BCO Bio-Rad GelDoc. See info below. -------- Original Message -------- Subject: Re: [Lbiok] New GelDoc instrument Date: Mon, 19 Mar 2012 10:57:37 +0000 From: Hongmin Tu <Hongmin.Tu(a)oulu.fi> To: lbiok(a)lists.oulu.fi <lbiok(a)lists.oulu.fi> Hi, Our new Bio-Rad GelDoc instrument is planned to be installed tomorrow, although it has not arrived here yet. The service engineer’s schedule is: 10:00-12:00, set-up the system 12:00-13:00, lunch 13:00-15:00, training, the first step is to get familiar with the instrument, and the second one is to learn the imaging software at L101A. For users of BCO old Bio-Rad GelDoc XR instrument, the spare parts for updating your machine have already arrived. There are 3 parts, i.e. filter for green fluorescence, screen safer + googles, and the new software. BRs, Hongmin

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Life Technologies - Andrew Howitt
by Teija Luoto 19 Mar '12

19 Mar '12

Dear All, Andrew Howitt will be on campus Monday afternoon (Aapistie 5A) if anyone wants to discuss products, pricing, promotions or concerns. He will be there on Tuesday on Biocenter Day 2012 exhibition as well. Andrew Howitt Sr. Inside Sales Representative, Nordics Consumables T +44 (0) 141 814 5751 . M +44 (0) 7506 039436 . F +44 (0) 141 814 3257 3 Fountain Drive . Paisley . Inchinnan Business Park . PA4 9RF . UK andrew.howitt(a)lifetech.com www.lifetechnologies.com ******************************** Teija Luoto, Secretary Biocenter Oulu, University of Oulu P.O. Box 5000 FI-90014 UNIVERSITY OF OULU, FINLAND Street address: Aapistie 5A, 90220 OULU, FINLAND Tel +358 8 537 6103, Fax +358 8 537 6115 e-mail: teija.luoto(a)oulu.fi BIOCENTER OULU URL: http://www.biocenter.oulu.fi (internal mail: 5BIOCENTER)

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TOMORROW - Biocenter Day 2012 - Tuesday 20.3.20120
by Teija Luoto 19 Mar '12

19 Mar '12

BIOCENTER OULU DAY 2012 Biological Dark Matter Cracking the Non-Coding Time Tuesday, March 20, 2012 Place Auditorium 101A in the Main Building of the Medical Campus, Aapistie 5A,University of Oulu, Oulu, Finland Transportation There will be bus transportation from Linnanmaa to Aapistie 5A. The bus will leave at 8.30 hrs at door J from Linnanmaa and return at 17.15 hrs from Aapistie 5A to Linnanmaa Chairpersons Madhura Patankar, Pauli Ohukainen and Mikko Vuorela PROGRAMME 09.00 09.05 Opening remarks Chairperson of the Organizing Committee, Biocenter Oulu 09.05 09.15 Welcome Prof. Johanna Myllyharju, Scientific Director of Biocenter Oulu 09.15 - 09.20 Welcome Prof. Seppo Vainio, Director of Biocenter Oulu Doctoral Programme 09.20 10.00 Small regulatory RNAs and their targets in Salmonella Dr. Kai Papenfort IMIB, University of Würzburg, Germany 10.00 10.35 Coffee at the exhibition area (35 min) 10.35 11.15 Finding the sequences that matter in non-coding RNAs: Evolutionary analysis coupled to functional characterization Dr. Mikko Frilander Institute of Biotechnology, University of Helsinki, Finland 11.15 11.55 Transcriptional dynamics of miRNA promoters and long noncoding RNAs Dr. Chirag Nepal HIB, Department of Molecular Biology, University of Bergen, Norway 11.55 13.25 Lunch break and exhibition (1 h 30 min) 13.25 14.05 Biochemical dissection of small RNA-guided gene silencing pathways Prof. Dr. Gunter Meister Biochemistry, University of Regensburg, Germany 14.05 14.45 The heat shock response: implications in cell stress and development Prof. Lea Sistonen Biocity, Åbo Akademi, Turku, Finland 14.45 15.25 Coffee at the exhibition area (40 min) 15.25 16.05 Control of lipid metabolism by miR-33 Prof. Carlos Fernandez-Hernando NYU Langone Medical Center, New York, USA 16.05 16.45 Epigenetic regulation of genes by small RNAs: a novel tool for gene therapy of cardiovascular disease Dr. Mikko Turunen Ark Therapeutics Oy, Kuopio, Finland 16.45 17.00 General discussion and concluding remarks ******************************** Teija Luoto, Secretary Biocenter Oulu, University of Oulu P.O. Box 5000 FI-90014 UNIVERSITY OF OULU, FINLAND Street address: Aapistie 5A, 90220 OULU, FINLAND Tel +358 8 537 6103, Fax +358 8 537 6115 e-mail: teija.luoto(a)oulu.fi BIOCENTER OULU URL: http://www.biocenter.oulu.fi (internal mail: 5BIOCENTER)

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Olympus FV1000 confocal repaired
by Veli-Pekka Ronkainen 15 Mar '12

15 Mar '12

Dear users, LM core facility's Olympus FluoView1000 confocal microscope is finally fully functional! The channel 1 background problem is fixed so Dapi, Hoechst etc. blue label quantitative imaging is possible again. Please report any possible anomalies to core personnel. Regards, Veli-Pekka Ronkainen BCO Light Microscopy Core Facility

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Bco-kieppi March 2012