Bco-kieppi April 2018

bco-kieppi@lists.oulu.fi

7 participants
14 discussions

EA kertauskurssille 29.5. on vielä tilaa
by Pirkko Huhtala 09 Apr '18

09 Apr '18

Hei, Only in Finnish: EA kertauskurssille 29.5. on vielä tilaa! Ilmoittautuminen henkilöstöportaalissa. Terv. Pirkko Pirkko Huhtala, Ph.D. Coordinator Biocenter Oulu University of Oulu Mailing address: P.O.Box 5000, FIN-90014 University of Oulu Finland Street address: Aapistie 5A, FIN-90220 Oulu Tel. sis/int. 486101, ulk./Ext. 0294 486101 Tel. +358-294 486101 e-mail pirkko.huhtala(a)oulu.fi<mailto:pirkko.huhtala@oulu.fi> www.oulu.fi/biocenter<http://www.oulu.fi/biocenter>

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Method Course on Immunohistochemistry, April 24-25
by Ritva Saastamoinen 04 Apr '18

04 Apr '18

Hi, Registration to the Method Course on Immunohistochemistry (April 24-25) is now open until April 17. The course info is available also in the HBS-DP web pages: see our calendar of events at http://www.oulu.fi/uniogs/dp/hbsdp/calendar-of-events. Here<http://www.oulu.fi/sites/default/files/content/Immunohisto%20Course%20Oulu%…> is the direct link to the programme pdf. Best wishes, Ritva *********** Method Course on Immunohistochemistry (1 ECTS), April 24-25, 2018 Venue: Biocenter Oulu histology lab 479B, Kontinkangas, Aapistie 5A, 90220 Oulu Teacher: Lotta Leveelahti, Histolab Products Oy Organizers: Health and Biosciences Doctoral Programme, Biocenter Oulu Transgenic Mouse Core Facility, and Histolab Products Oy Credits: 1.0 ECTS for field-specific studies in doctoral training Enquiries: Dr. Reetta Vuolteenaho, reetta.vuolteenaho(at)oulu.fi Registration: The course is limited to about 12 participants selected by application. Priority is given to doctoral students. Online registration/application, deadline April 17. All applicants will be notified on April 18. This 2-day practical course includes short lectures on each step of the immunohistochemistry (IHC) protocol. Additionally, you get hands-on experience about the micropolymer detection method with human tissue samples. Micropolymer detection enables superior sensitivity and reduced background staining compared to the traditional avidin-biotin-based detection. In the course, we will this time perform simultaneous chromogenic multiplex staining on a paraffin section. Is it as easy as doing a single stain? Come and see! The course participants will do single stainings using their own mouse tissue sections (3-4 slides) and their own primary antibody which has been tested for immunohistochemistry. The human tissue samples, secondary antibodies and reagents for double stainings will be provided by the organizers. Apply online: https://www.webropolsurveys.com/S/24D17892DA717B7B.par Course programme Day 1 (9.00-15.00) 9.00-10.00 Intro IHC + Pre-treatments + Blocking 10.00-10.15 Lab: Deparaffinisation and start pre-treatments 10.15 - 10.30 Break 10.30-11.00 Antibodies 11.00-11.15 Lab: Slides into TBS 11.15 - 11.45 Detection 11.45-12.00 Lab: Background blocking and application of antibody 12.00-13.00 Lunch break 13.00 - 13.15 Lab: Application of Probe 13.15 - 13.30 Chromogen + counterstaining + Mounting 13.30 - 13.45 Lab: Application of Polymer 13.45 - 14.15 Trouble shooting + Wrap Up 14.15 - 15.00 Lab: Application of Chromogen + Counter staining + Mounting + Check results Day 2 (9.00-12.45) 09.00 -09.20 Lab: Deparaffinisation and start pre-treatments 09.20 - 10.00 Multiplex IHC 10.00 -10.05 Lab: Slides into TBS 10.05-10.15 Break 10.15-10.30 Lab: Background blocking and application of antibody cocktail 10.30 - 11.00 Trouble shooting 11.00 - 11.10 Lab: application of polymer cocktail 11.10 - 11.40 Lunch break 11.40 - 12.00 Lab: Application of Chromogen 12.00 - 12.15 Questions and discussions 12.15 - 12.45 Counterstaining+ Mounting + check results **************************************************************** Ritva Saastamoinen, PhD, Docent Coordinator, Adjunct associate professor Health and Biosciences Doctoral Programme (HBS-DP) Biocenter Oulu<http://www.oulu.fi/biocenter/> and University of Oulu Graduate School<http://www.oulu.fi/uniogs/> P.O.Box 5000, FIN-90014 University of Oulu, Finland (Visiting address: Aapistie 5A, Room 473B) ritva.saastamoinen(at)oulu.fi Tel: +358 294 48 6102, mobile +358 50 526 7164 http://www.oulu.fi/uniogs/dp/hbsdp ****************************************************************

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Aapistie 5b sähkökatko huomenna 4.4.2018 klo.17.00-18.00
by Juhani Vänttilä 03 Apr '18

03 Apr '18

Tiedoksi! Sähkökatko vaikuttaa koko Aapistie 5B rakennukseen (Patologia, Koe-eläinkeskus, Farmakologia, Fysiologia, Elite) Patologianlaitoksen sähkökatko on huomenna keskiviikkona 4.4.2018 klo 17.00 - 18.00. Sähkökatkoon liittyen: Alueen kaksi varavoimakonetta ovat toiminnassa sähkökatkon aikana. Kulunvalvontajärjestelmät toimivat normaalisti sähkökatkossa akustojen avulla. Alueen jakamoiden aktiivilaitteiden sähkönsyöttö katkeaa ja tietoliikenneverkko menee alas niiltä osin. Tietokoneet ja palvelimet kannattaa ajaa alas ja irrottaa sähköverkosta ennen sähkökatkoa mikäli ne eivät ole UPSin perässä. Alueen hissit otetaan pois käytöstä ennen sähkökatkoa noin klo 16:30. Alueen pääkulkuväylien oviin kiinnitetään tiedote sähkökatkosta. Ystävällisin terveisin Raimo Kursula Projektipäällikkö Kiinteistöprojektinjohto P 040 744 9894 raimo.kursula(a)ramboll.fi<mailto:raimo.kursula@ramboll.fi> ________________________________________ Ramboll Kiviharjunlenkki 1A 90220 OULU www.ramboll.fi<http://www.ramboll.fi/>

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Thank you!
by Ulla Saarela 03 Apr '18

03 Apr '18

Dear colleagues, I want to thank everybody for remembering me on my doctoral dissertation day. Best regards, Ulla

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Bco-kieppi April 2018