Dear all,
This year’s workshop will be arranged by Dr. Stefan Prost and Dr. Marko Prous on 8.12.2022 at 17.30 in room B123 at Linnanmaa campus. Please find the abstract of the workshop and registration link below.
Miniature sequencing devices by Oxford Nanopore Technologies (ONT) have made sequencing of DNA and RNA easier than ever before, but it is still complicated enough for beginners, especially data processing, analysis and interpretation. Although the sequencing devices are small (USB-stick or smartphone size), additional equipment and reagents are needed to prepare the DNA or RNA into sequenceable form, filling up at least a suitcase. Nevertheless, successful field sequencing has already been demonstrated for ONT devices. In the workshop, we'll demonstrate how to use ONT devices using both small lectures as well as lab demonstrations. We will outline how libraries are prepared, demonstrate the loading and sequencing using ONT’s MinION MK1C devise, go through the basics of monitoring sequencing progress, discuss the basic steps of basecalling (translation of raw signal into sequences) and demultiplexing, and then demonstrate the aspects of the data analyses.
Registration link: https://docs.google.com/forms/d/1Gmw7QsRDwhTrBJ7hgaSsglkttLbDKN05ycX9EZit26c/
| The workshop of the 26th Kaamos Symposium will be arranged by Dr. Stefan Prost and Dr. Marko Prous on 8.12.2022 at 17.30 in room B123 at Linnanmaa campus. Workshop description: Miniature sequencing devices by Oxford Nanopore Technologies (ONT) have made sequencing of DNA and RNA easier than ever before, but it is still complicated enough for beginners, especially data processing, analysis and interpretation. Although the sequencing devices are small (USB-stick or smartphone size), additional equipment and reagents are needed to prepare the DNA or RNA into sequenceable form, filling up at least a suitcase. Nevertheless, successful field sequencing has already been demonstrated for ONT devices. In the workshop, we'll demonstrate how to use ONT devices using both small lectures as well as lab demonstrations. We will outline how libraries are prepared, demonstrate the loading and sequencing using ONT’s MinION MK1C devise, go through the basics of monitoring sequencing progress, discuss the basic steps of baseca |
Best wishes,
Kaamos Committee
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Kaamos Symposium 2022
8-9th December, 2022
Saalasti Hall,
University of Oulu
90570 Oulu, Finland
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