Biocenter Oulu is organizing the 4th workshop on

VISUALISATION OF CELLS AND TISSUES BY LIGHT AND ELECTRON MICROSCOPIC TECHNIQUES AND TOMOGRAPHIC METHODS

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Place                           Auditorium F101 of the Department of Physiology and the  Biocenter Oulu Laboratories of the Main Building of the Medical Campus,  University of Oulu, Aapistie 5A, P.O.Box 5000, FIN-90014 Oulu, Finland

Time                            24^th to 26^th October, 2007

Credits                         1.5 ECTS units for lectures and demonstrations 1 to 4; 0.3 ECTS units for lectures only.

Organisers                  Biocenter Oulu Core Facilities at the BCO Tissue Imaging Center and the Departments of Medical Biochemistry and Molecular Biology and Pathology, University of Oulu, P.O. Box 5000, FIN-90014  University of Oulu, Finland

Aim                            To get acquainted with modern imaging technologies  and collecting of images of living and fixed cultured cells as well as tissues using video microscope, laser scanning confocal microscope,  electron microscope and optical tomography methods

Information                Sinikka Eskelinen, Biocenter Oulu, Aapistie  5A, FIN-90014 University of Oulu, Finland, tel. 358-8-537 6101, fax 358-8-537 6115, e-mail sinikka.eskelinen@oulu.fi

URL: http://www.biocenter.oulu.fi/

 

Registration    Registration for lectures recommended, registration for the demonstrations obligatory.  Only 16 students are accepted for demonstrations.     The  application with a short description of the research project should be sent to Sinikka Eskelinen by September 28, 2007. Preference will be given to students whose projects best benefit the topics of the demonstrations given during the course.

 

PROGRAM

 

Wednesday,  October 24^th, 2007, Auditorium F101 of the Department of Physiology of the Medical Campus, Aapistie 7

Chair persons: Sinikka Eskelinen, Raija Sormunen,  Aki Manninen

 

9.00 - 9.15 Opening words  by Sinikka Eskelinen

 

9.15  - 9.45 Live-cell imaging with EosFP and other photoactivatable marker proteins of the GFP family

Jörg Wiedenmann, School of Ocean & Earth Science, <?xml:namespace prefix = st1 ns = "urn:schemas-microsoft-com:office:smarttags" />University of Southampton National Oceanography Centre, Southampton European Way, SOUTHAMPTON, U.K.

 

9.45 – 10.15  Live cell imaging/News from Olympus

Aki Manninen, Biocenter Oulu/Representative from Olympus

 

10.15 - 11.15 Imaging Intracellular Signalling

Michael Courtney, Molecular Signalling Laboratory, Department of Neurobiology,  A.I. Virtanen Institute, University of Kuopio, Finland

11.15 - 12.30               Lunch

 

12.30 - 13.30  Optical projection tomography: 3D microscopy of embryos and organs

Jamie Davies, University of Edinburgh, Edinburgh, Scotland, U.K.

                                                                       

13.30 - 14.30  Confocal microscopy of thick specimens

Nathalie Garin, Microscopy, Imaging & Morphology Facility, ISREC, Swiss Federal Institute of Technology in Lausanne, Switzerland

 

14.30 - 15.00               Coffee

 

15.00 - 16.00   Electron microscopic 3D imaging

Willie Geerts, Electron Microscopy Group Utrecht, University of Utrecth, The Netherlands

16:00 – 16.15 Concluding remarks  by Raija Sormunen

 

 

Thursday and Friday, October 25th to 26th , 2007

 

There will be four groups of four students participating in the demonstrations, two demonstrations per day. 

 

Demonstrations:

1. Olympus confocal microscope 1: visualisation of thick specimens,   room 423A

Teacher: Nathalie Garin, Aki Manninen

2. Olympus confocal microscope 2 equipped with second laser: photoactivatable marker proteins in living cells, room 422A

Teacher: Jörg Wiedenmann, Aki Manninen

3.  Live cell imaging 1 using Olympus inverted microscope and Okolab chamber: long term cell culture and wound healing, room 421A

Teacher: Terhi Teräväinen and Riikka Wirkkala

Live cell imaging 2 using Olympus Cell M system: visualisation of cell organelles and cytoskeletal components in living cells, room 419A
Teacher: Sinikka Eskelinen and Linda Rosberg

4. Electron microscopy: 3D tomography of EM images,  room 337B (microcomputer class)

Teacher:  Willie Geerts
 
 
Sinikka Eskelinen, Ph.D.
Coordinator
Biocenter Oulu
University of Oulu
mailing address:
P.O.Box 5000, FIN-90014 University of Oulu
Finland
street address:
Aapistie 5A, FIN-90220 Oulu
Tel. 358-8-5376101
Fax 358-8-5376115
e-mail sinikka.eskelinen@oulu.fi
BIOCENTER URL:http://www.biocenter.oulu.fi

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