BASIC COURSE ON LIGHT AND ELECTRON MICROSCOPY
"Imaging across the scales, from electron microscopy to mesoscopic imaging"
Time Feb 1st to Feb 3rd, 2023
Place BCO Light Microscopy (LM) and Electron Microscopy (EM) Core Facility Laboratories, the Main Building of the Medical Campus, University of Oulu, Aapistie 5A, 90220 Oulu, Finland
Credits 1 ETCS
Organisers Health and Biosciences Doctoral Programme and Oulu BioImaging, Biocenter Oulu, University of Oulu, https://www.oulu.fi/en/university/faculties-and-units/biocenter-oulu
Aim To get acquainted with light and electron microscopy imaging methods of living and fixed specimens of different sizes. Learn how to apply suitable techniques for image analysis. LM introduction includes widefield and confocal fluorescence microscopy and examples of multiphoton techniques. EM part focuses on sample preparation and transmission and scanning electron microscopy. The course consists of lectures, microscopy demonstrations and computer-based image analysis workshops.
Information Veli-Pekka Ronkainen / Ilkka Miinalainen, Biocenter Oulu, tel: +358 294 486114 / +358 294 486145, e-mail: veli-pekka.ronkainen(at)oulu.fimailto:veli-pekka.ronkainen@oulu.fi / ilkka.miinalainen(at)oulu.fi
Registrationhttps://link.webropolsurveys.com/S/393F761F20194ABC Lectures are open for all without registration. Registration for the demonstrations is obligatory and preference will be given to Ph.D. students and researchers whose project benefits the topics carried out during the course. Due to space limitations only 18 participants can be accepted. The application with a short description of the applicant's project should be submitted via Webropol linkhttps://link.webropolsurveys.com/S/393F761F20194ABC by 25th of January 2023. Selected applicants will be informed by Jan 26th.
PROGRAMME
Wednesday, Feb 1st, 2023. Lecture hall P107 (Pathology)
Lectures:
9.00-9.05 Opening words and introduction
9.05-10.00 Basics in light/fluorescence microscopy Veli-Pekka Ronkainen, BCO
10.00-10.15 Coffee break
10.15-11.15 Basics in electron microscopy
Ilkka Miinalainen, BCO
11.15-12.15 Lunch break
12.15-13.15 Principles of advanced fluorescence microscopy techniques
Veli-Pekka Ronkainen 13.15-13.30 Break
13.30-14.30 Methods for preparing biological samples for electron microscopy analysis
Ilkka Miinalainen
14.30-14.45 Introduction to demonstrations
Veli-Pekka Ronkainen
Thursday, Feb 2nd, 2023
8.45 Gathering in meeting room 487B (Kieppi Building 4th floor, Aapistie 5A) for demonstrations.
9.00-16.00 Demonstrations, 3 groups
Demo 1. Laser scanning confocal and multiphoton microscopy, Room 423A and 492B
Imaging of cells and 3D imaging of thick mesoscopic samples, Veli-Pekka Ronkainen
Demo 2. Transmission electron microscopy and sample preparation, Room 467B and 488B
Imaging of tissue samples and negative staining and imaging of macromolecules,
Mika Kaakinen
Demo 3. Scanning electron microscopy and sample preparation, Room 320A and 488B
Imaging of tissue and cell samples using different detectors and preparing samples for sputter coating, Ilkka Miinalainen
Hours
1st group
2nd group
3rd group
9.00-11.00
Demonstration 1
Demonstration 2
Demonstration 3
11.00-12.00
Lunch break
Lunch break
Lunch break
12.00-14.00
Demonstration 2
Demonstration 3
Demonstration 1
14.00-16.00
Demonstration 3
Demonstration 1
Demonstration 2
Friday, Feb 3rd, 2023. Room 289B and Computer Class KT285
9.00-10.00 Introduction to computer-based image processing and analysis, Room 289B,
Mika Kaakinen, BCO
10.00-10.15 Coffee break
10.15-11.45 Computer-based processing and analysis of light microscopy images, Computer Class KT285
Veli-Pekka Ronkainen, Antti Viklund, Mika Kaakinen
11.45-12.45 Lunch break
12.45-14.15 Computer-based processing and analysis of electron microscopy images, Computer Class KT285, Ilkka Miinalainen, Antti Viklund, Mika Kaakinen
14.15 Concluding remarks
Best wishes, Mirja ------------------------------------------------------------------------------------------------------ Mirja Peltola, PhD, BSc (Tech.) Koordinaattori | Coordinator Oulun yliopisto | University of Oulu Terveys- ja biotieteiden tohtoriohjelma | Health and Biosciences Doctoral Programme Tel. 0294 48 5447 PO Box 5000, FI-90014 OULUN YLIOPISTO (Aapistie 5A, Room 476B) www.oulu.fihttp://www.oulu.fi/yliopisto
www: Terveys- ja biotieteiden tohtoriohjelma | Health and Biosciences Doctoral Programmehttps://www.oulu.fi/en/science-arctic-attitude/graduate-school/organisation-and-contact-information-uniogs/health-and-biosciences-doctoral-programme