Subject: [Ltkhkunta] Basic course on light microscopy, June 11-13, Oulu

BIOCENTER OULU, Light Microscopy Core Facility Course

BASIC COURSE ON LIGHT MICROSCOPY

Place             Auditorium P117 and BCO Light Microscopy Core Facility Laboratories, the Main Building of the Medical Campus, University of Oulu, Aapistie 5A, 90220 Oulu, Finland

Time              11^th to 13^th June, 2014

Credits          1 ETCS

Organisers    Biocenter Oulu Doctoral Programme and Biocenter Oulu Tissue Imaging Center, University of Oulu, Aapistie 5A, P.O.Box 5000, FI-90014 University of Oulu, Finland, URL: http://www.oulu.fi/biocenter/tic

Aim               To get acquainted with light and fluorescence imaging methods of living and fixed microscopic specimens. Learn how to apply suitable techniques for analysis of images and time lapse movies. Introduction includes basic microscopy, laser scanning and spinning disc confocal microscopy and light sheet fluorescence microscopy.

Information Veli-Pekka Ronkainen, Biocenter Oulu, Aapistie 5A, P.O.Box 5000, FI-90014, University of Oulu, Finland, tel. +358 294 486114, e-mail: veli-pekka.ronkainen(at)oulu.fi

The course consists of lectures, independent learning, microscopy demonstrations and computer-based image analysis.

Registration Lectures are open for all without registration. Registration for the demonstrations is obligatory and preference will be given to Ph.D. students and researchers whose project benefits the topics carried out during the course. Due to space limitations only 18 participants can be accepted. The application with a short description of the applicant’s project should be sent by email to Veli-Pekka Ronkainen (veli-pekka.ronkainen(at)oulu.fi) by 23^th May, 2014.

PROGRAMME

Wednesday, June 11^th, 2014. Auditorium P117 (Pathology) 

Lectures:

9.00-9.05           Opening words and introduction

                             Sinikka Eskelinen, Biocenter Oulu

9.05-10.00        Basics in light/fluorescence microscopy

Sinikka Eskelinen

10.00-10.15      Coffee break

10.15-11.00      Confocal microscopy techniques

Veli-Pekka Ronkainen, Biocenter Oulu

11.00-11.15      Break

11.15-12.15      Challenges in live cell imaging

Group work in 2-3 person groups

Veli-Pekka Ronkainen, Janne Capra and Fumi Suomi, Biocenter Oulu

12.15-13.15      Lunch

13.15-14.00      Principles of light sheet fluorescence microscopy

                             Veli-Pekka Ronkainen

14.00-14.15      Break

14.15-15.15      Examples of practical applications utilizing light microscopy 

                             Invited speakers from local research groups, short talks

15.15-16.00      Introduction to demonstrations

                             Veli-Pekka Ronkainen

Thursday, June 12^th, 2014. Gathering in the meeting room 487B, Biocenter Oulu 4^th Floor, Aapistie 5A at 8.45 a.m. for demonstrations.

9.00-16.00        Demonstrations, 3 groups

Demo 1.            Laser scanning confocal microscopy, Room 421A

Imaging of hypoxic living cells and 3D imaging of thick specimens

                             Veli-Pekka Ronkainen, Biocenter Oulu

Demo 2.            Spinning disc confocal microscopy, Room 422A

Live cell fluorescence and time lapse overnight imaging, molecular dynamics (FRAP)

Fumi Suomi, Biocenter Oulu

Demo 3.            Imaging colocalization with a confocal microscope, Room 423A

Imaging and visualization of colocalization in multi-labelled samples

Janne Capra, Biocenter Oulu

Hours	1^st group	2^nd group	3^rd group
9.00-11.00	Demonstration 1	Demonstration 2	Demonstration 3
11.00-12.00	Lunch	Lunch	Lunch
12.00-14.00	Demonstration 2	Demonstration 3	Demonstration 1
14.00-16.00	Demonstration 3	Demonstration 1	Demonstration 2

Friday, June 13^th, 2014. Meeting Room 487B, Biocenter Oulu 4^th Floor / Computer Class KT285

9.00-10.00        Computer-based image processing and analysis, Meeting Room 487B

Basic introduction to image processing

Neslihan Bayramoglu, Center for machine vision research, University of Oulu

Segmentation of fluorescently labeled cells in 3D matrix,

Saad Ullah Akram, Center for machine vision research, University of Oulu

10.00-10.15      Coffee break

10.15-11.00      Computer-based processing and analysis of captured images and time lapse movies, Computer Class KT285

Veli-Pekka Ronkainen, Antti Viklund, Fumi Suomi

11.00-12.00      Lunch

12.00-14.00      Computer-based processing and analysis of captured images and time lapse movies continues, Computer Class KT285

Veli-Pekka Ronkainen, Antti Viklund, Fumi Suomi

14.00                  Concluding remarks, Veli-Pekka Ronkainen

Course programme available at http://www.oulu.fi/sites/default/files/content/Basic_microscopy_course_June_2014.pdf . It can be found also via the Biocenter Oulu front page under “Events”, BCO Calendar of events and the Oulu BioImaging education page.

If you have any questions, please contact Dr. Veli-Pekka Ronkainen, tel. +358 294 486114, e-mail: veli-pekka.ronkainen(at)oulu.fi.

Kind regards,

Ritva

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Ritva Saastamoinen, PhD, Docent

Biocenter Oulu Doctoral Programme Coordinator

Adjunct associate professor

Biocenter Oulu

PL 5000 (Aapistie 5a)

FIN-90014 University of Oulu, Finland

Tel: (+358) 0294 48 6102

ritva.saastamoinen(at)oulu.fi 

http://www.oulu.fi/biocenter/doctoral-programme

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