TISSUE ANALYSIS COURSEhttps://www.oulu.fi/en/tissue-analysis-course-2023 https://www.oulu.fi/en/tissue-analysis-course-2023
DP00AL85-3001, 1-3 ECTS
Time: 17.3. - 23.3.2023
Place: Kontinkangas Campus
Target group: Doctoral students in the fields of Health and Biosciences, lectures open for all
Credits: 3 ECTS (participation to all lectures, demonstrations and exercises, providing feedback) ; 1 ECTS (participation to all lectures*, providing feedback)
Teaching language: English
Course enrolment: Registration via Peppi https://www.oulu.fi/en/peppi by 24th February 2023, course code: DP00AL85-3001
Acceptance: 12 participants (incl. demonstrations, practical work and exercises) selected on account of a motivation letter
Evaluation criteria: Passed/failed according to participation in lectures and demonstrations, carrying out practical work and exercises, providing feedback
Organizers: Biocenter Oulu, Biobank Borealis of Northern Finland and Research Units of Translational Medicine and Clinical Medicine
Contact information: anne.heikkinen(at)oulu.fi, reetta.vuolteenaho(at)oulu.fi, Biocenter Oulu Transgenic and Tissue Phenotyping Core Facility (BCO TTP Core), tel: +358-(0)294 486 133
Programme: Friday 17th March, 2023 Lecture room 337B/338B, lectures* 8:00-8:15 Introduction to the course* 8:15-9:15 Mouse as a model for human diseases* Reetta Hinttala, BCO TTP Core and RU of Clinical Medicine 9:15-9:30 Coffee break 9:30-10:30 From tissue to biomedical sample* Anne Heikkinen, BCO TTP Core 10:30-11:00 Introduction to mouse necropsy* Miia Salo, BCO TTP Core Histology Lab 479B, practical work 11:00-11:30 Printing of tissue cassettes TTP Core personnel 11:30-12:15 Lunch break, after which gathering in the Kieppi lobby Oulu Laboratory Animal Centre 12:15-16:00 Mouse necropsy Miia Salo and Anne Heikkinen, BCO TTP Core
Monday 20th March, 2023 Histology Lab 479B, practical work 8:00-9:15 Paraffin embedding Objective slide printing TTP Core personnel Seminar room 487B, lecture* 9:15-10:45 Sample preparation for electron microscopy* Introduction to the Electron Microscopy Image Library* Ilkka Miinalainen, BCO EM Core 10:45-11:30 Lunch break Histology Lab 479B, practical work 11:30-16:00 Paraffin block sectioning Cryo sectioning Manual HE staining of cryo sections Whole mount sample preparation BCO TTP Core personnel Tuesday 21st March, 2023 Histology Lab 479B, demonstration 8:00-9:00 Automated HE staining BCO TTP Core personnel Lecture room P107, lecture 9:00-10:00 Common histological staining methods and artefacts Markus Mäkinen, RU of Translational Medicine 10:00-10:15 Coffee break 10:15-11:15 Basic histological structures and findings Markus Mäkinen, RU of Translational Medicine Histology Lab 479B, demonstration 11:15-11:45 Automated slide cover slipping TTP Core personnel 11:45-12:30 Lunch break Lecture room P107, lecture* 12:30-13:30 Principles of IHC and In situ hybridization methods* Katja Porvari, Faculty of Medicine Laboratory Unit 13:30-14:00 Possibilities in utilizing Biobank samples in research* Raisa Serpi, Biobank Borealis 14:00-14:15 Coffee break, after which gathering in the Kieppi lobby Pathology Laboratory of the RU of Translational Medicine, demonstration 14:15-15:30 Tour through a Pathology Laboratory Demonstration on automated IHC and In situ hybridization Markus Mäkinen, RU of Translational Medicine and Katja Porvari, Faculty of Medicine Laboratory Unit Wednesday 22nd March, 2023 Independent studies Familiarizing with preannounced web pages, such as EM Library<fingmice.fi/em-library> Familiarizing with and preparing for QuPath image analysis exercises Thursday 23rd March, 2023 Histology Lab 479B, demonstration/practical work 8:00-9:00 Microscopy of histological slides Veli-Pekka Ronkainen, BCO LM Core Automated slide scanning TTP Core personnel Computer class 286A/286B, lecture* 9:00-10:00 Basics in image analysis* Veli-Pekka Ronkainen, BCO LM Core 10:00-10:15 Coffee break 10:15-11:15 Prospects of the open-access QuPath image analysis tool* Juha Väyrynen, RU of Translational Medicine 11:15-12:00 Lunch break 12:00-13:00 The Visiopharm image analysis software features* Anne Heikkinen, BCO TTP Core
Computer class 286A/286B, practical work/demonstration 13:00-16:00 Exercises with QuPath Juha Väyrynen and Sanna Palosaari, RU of Translational Medicine Demonstration on the Visiopharm software working principles Anne Heikkinen and Miia Salo, BCO TTP Core
Best wishes, Mirja
------------------------------------------------------------------------------------------------------ Mirja Peltola, PhD, BSc (Tech.) Koordinaattori | Coordinator Oulun yliopisto | University of Oulu Terveys- ja biotieteiden tohtoriohjelma | Health and Biosciences Doctoral Programme Tel. 0294 48 5447 PO Box 5000, FI-90014 OULUN YLIOPISTO (Aapistie 5A, Room 476B) www.oulu.fihttp://www.oulu.fi/yliopisto
www: Terveys- ja biotieteiden tohtoriohjelma | Health and Biosciences Doctoral Programmehttps://www.oulu.fi/en/science-arctic-attitude/graduate-school/organisation-and-contact-information-uniogs/health-and-biosciences-doctoral-programme