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Specialised phenotyping / metabolic phenotyping pipeline Call information and application form
Context and aim of the call INFRAFRONTIER is the European Research Infrastructure for phenotyping and archiving of model mammalian genomes. The INFRAFRONTIER Research Infrastructure provides access to first-class tools and data for biomedical research, and thereby contributes to improving the understanding of gene function in human health and disease using the mouse model. The core services of INFRAFRONTIER comprise the systemic phenotyping of mouse mutants in the participating mouse clinics, and the archiving and distribution of mouse mutant lines by the European Mouse Mutant Archive (EMMA).
Main objective of this INFRAFRONTIER2020 Trans-national Access call is to facilitate access for the wider biomedical research community to the unique infrastructure and scientific expertise of the participating INFRAFRONTIER mouse clinic, where mouse mutant lines can be tested through phenotyping pipelines with special focus on metabolic functions. As an example, disturbed energy balance regulation results in obesity and in numerous related metabolic disorders such as type 2 diabetes mellitus. We offer specialised phenotyping pipelines comprising state-of-the-art test assays monitoring disease related parameters.
Access will be granted based on scientific excellence and supports the development and in-depth characterisation of mouse models for investigating gene function and human pathophysiology. INFRAFRONTIER will provide open access to all characterised disease models and phenotyping data.
During the course of the INFRAFRONTIER2020 project two further Trans-national Access calls supporting 12 specialised phenotyping projects will be published. One further call with a focus on a functional immune-phenotyping screen by mass cytometry, and on induced secondary phenotyping screen under acute or more chronic inflammatory conditions will be released in spring 2019.
More information and call application form: https://www.infrafrontier.eu/resources-and-services/infrafrontier-open-calls... Proposal submission to proposals@infrafrontier.eumailto:proposal@infrafrontier.eu by 15th April 2019. Proposal evaluation from 15 April 2019 to 15 May 2019. The INFRAFRONTIER2020 project has received funding from the EU Research and Innovation programme Horizon 2020 (H2020-EU.1.4.1.1. Developing new world class research infrastructures) www.infrafrontier.euhttps://www.infrafrontier.eu/ and https://twitter.com/InfrafrontierEU
Trans-national Access (TA) activity of the INFRAFRONTIER2020 project Free of charge specialised phenotyping service / Access modalities
· The EC Horizon 2020 funded INFRAFRONTIER2020 project (2017 – 2020) supports eligible customers with a free-of-charge mouse model specialised phenotyping service implemented as a Trans-national Access activity supporting a total of 3 projects in this call. · The access unit offered covers the specialised metabolic phenotyping of a mouse mutant line, and the preparation of a comprehensive phenotyping report. · Participating mouse clinic is the Czech Centre for Phenogenomics (CCP), IMG AS CR v.v.i. · Centre specific collaboration agreements will be established between applicants and the mouse clinic. · Starting material for the phenotyping projects is a breeding nucleus and then cohorts are bred by the CCP. Mouse mutants from various sources (transgenes, knockout mice, mutants from mutagenesis screens like ENU) and of different genetic backgrounds can be accepted. · The phenotyping pipeline to be used will be a specialised metabolic phenotyping pipeline based on published IMPReSS SOPs (IMPReSS:http://www.mousephenotype.org/impress), or on local mouse clinic SOPs. Details of the CCP metabolic phenotyping pipeline are described on page 5 of this application form. · Support will be provided by the CCP to analyse and interpret the phenotyping data. · The analysed mouse models and the generated phenotyping data will be made available to the scientific community. An optional grace period of up to 1 year for mouse resources and phenotype data may apply, with immediate release of mouse resources and data after expiry of the grace period. A phenotyping report with all phenotyping data will be prepared, and data will also be uploaded onto a public phenotype database of the mouse clinics and / or onto the IMPC portal at http://www.mousephenotype.org. Mouse mutant lines will be deposited into the INFRAFRONTIER/EMMA repository for subsequent use by the scientific community, and will be distributed using the applicant’s institutional MTAs. · Costs: The access to the INFRAFRONTIER2020 phenotyping service is free of charge. However, the shipment cost of mouse mutant lines to the CCP in Prague must be borne by the applicants. · Eligibility: The INFRAFRONTIER2020 Trans-national Access call is open and proposals can be submitted from applicants around the world. · Application: Requests for the INFRAFRONTIER2020 specialised phenotyping service can be made via this application form. Applications for the Trans-national Access activity must include a short description of the research plans for utilising the phenotyped mouse model that is being characterised by the INFRAFRONTIER2020 TA service. · Selection procedure: Proposals from eligible applicants for free of charge access to the INFRAFRONTIER2020 specialised phenotyping service will be subject to a review procedure. The review will be based on short descriptions of the projects involving the mouse mutants that will be phenotyped by the TA service. A mixed panel of members of INFRAFRONTIER and of an external Evaluation Committee will assess service requests supported by the TA activity. In addition to scientific merit of applicants, relevance and quality of preliminary data, soundness of the proposal and research plans, and the project objectives and prospects for exploitation of phenotype data will be assessed. In a further step experts of the mouse clinics will assess the technical feasibility of projects. The technical evaluation of projects may require the provision of additional data such as: -Information on the genetic modification of your mutant mouse line if applicable (e.g. affected gene, MGI ID of the gene, type of mutation, ES-cell line used, genetic background (e.g. number of backcross generations) -Description of DNA modification (vector, remaining non-recipient DNA, donor organism) -Mutant phenotype(s), special housing or care requirements -Current sanitary status -Intellectual property rights (who generated and who owns the mouse line) Applicants will be informed on the outcome of the evaluation within 4 weeks after the end of the call for which the TA application was submitted. All applications will be handled with strict confidentiality. · Acknowledgements: Please do acknowledge any support under this scheme in all resulting publications with ‘Part of this work has been funded by the European Union Research and Innovation programme Horizon 2020 (Grant Agreement Number 730879)’, and by LM2015040 (Czech Centre for Phenogenomics), CZ.1.05/2.1.00/19.0395, and CZ.105/1.1.00/02.0109 funded by the Ministry of Education, Youth and Sports and the European Regional Development Fund. The participating infrastructure, which provided the service, should be specifically mentioned in any publication resulting from the service.
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