Subject: [Ltkhkunta] 6th WORKSHOP on Visualisation of Cells and Tissues by Light Microscopic Techniques and Tomographic Methods, June 12-14, Oulu

 

Hi everyone,

 

Biocenter Oulu Doctoral Programme (BCO-DP) and Biocenter Oulu Tissue Imaging Center are organizing a workshop on Visualisation of Cells and Tissues by Light Microscopic Techniques and Tomographic Methods. The course will present lectures to Ph.D. students and all scientists interested in the latest advanced microscopic and mesoscopic imaging methods. Demonstrations are limited to Ph.D. students and researchers whose projects benefit the topics carried out during the course.

 

Teachers of the course are pioneering experts in the field. Jan Huisken (Max Planck Institute, Dresden, Germany) has been developing SPIM technique and used it very successfully for imaging thick biological specimens, Bettina Weigelin (Radboud University Nijmegen, The Netherlands) has developed methods for tumor intravital- and 3D organotypic imaging, Ulf Ahlgren (University of Umeå, Sweden) is an expert in optical projection tomography methods for developmental biology applications and Pasi Kankaanpää (University of Turku, Finland) is one of the creators of a novel open-source image processing and analysis software BioImageXD.

 

Please find the detailed programme and more information from below and via this link: http://www.oulu.fi/sites/default/files/content/Advanced_microscopy_course_June_2013.pdf .

 

Kind Regards,

Ritva

 

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6th WORKSHOP on Visualisation of Cells and Tissues by Light Microscopic Techniques and Tomographic Methods

 

 

 

 

Place             Auditorium F202 and BCO Light Microscopy Core Facility Laboratories, the Main Building of the Medical Campus, University of Oulu, Aapistie 5A, 90220 Oulu, Finland

Time              12th to 14th June, 2013

Credits          1 ETCS

Organisers    Biocenter Oulu Doctoral Programme and Biocenter Oulu Tissue Imaging Center, University of Oulu, Aapistie 5A, P.O.Box 5000, FIN-90014 University of Oulu, Finland, URL: http://www.oulu.fi/biocenter/tic

Aim               To get acquainted with light and fluorescence imaging methods of living and fixed microscopic and mesoscopic scale specimens using laser scanning confocal microscopy, light sheet microscopy and optical projection tomography. Learn how to apply suitable techniques for analysis of microscopic images and time lapse movies.

Information Veli-Pekka Ronkainen, Biocenter Oulu, Aapistie 5A, P.O.Box 5000, FIN-90014, University of Oulu, Finland, tel. +358 294 486114, e-mail: veli-pekka.ronkainen(at)oulu.fi

The course consists of lectures, independent learning, microscopy demonstrations and computer-based image analysis.

 

Registration Lectures are open for all without registration. Registration for the demonstrations is obligatory and preference will be given to Ph.D. students and researchers whose project benefits the topics carried out during the course. Due to space limitations only 18 participants can be accepted. The application with a short description of the applicant’s project should be sent by email to Veli-Pekka Ronkainen (veli-pekka.ronkainen(at)oulu.fi) by 24th May, 2013.

 

 

PROGRAMME

Wednesday, June 12th, 2013. Auditorium F202 (Pharmacology), The Main Building of the Medical Campus, Aapistie 5A

 

Lectures:

9.00-9.05           Opening words and introduction

                             Sinikka Eskelinen/Veli-Pekka Ronkainen, Biocenter Oulu, University of Oulu, Finland

 

9.05-10.35        Confocal-, multiphoton- and higher harmonic generation microscopy to visualize microanatomy and dynamic processes in live tissues

Bettina Weigelin, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen, The Netherlands

 

10.35-10.45      Coffee break

 

10.45-12.15      Optical Projection Tomography imaging of embryos and organs - studies from pancreas development and diabetes disease aetiology

Ulf Ahlgren and Christoffer Nord, Umeå Centre for Molecular Medicine, University of Umeå, Sweden

 

12.15-13.00      Lunch

13.00-14.30      Principles and applications of light-sheet microscopy

Jan Huisken, Max-Planck-Institute of Molecular Cell Biology and Genetics, Dresden, Germany

 

14.30-14.45      Coffee break

 

14.45-15.15      Commercial Light Sheet Z.1 Microscope for biological specimens

                             Product Manager, Carl Zeiss, Germany

 

15.15-16.00      Introduction to demonstrations

                             Veli-Pekka Ronkainen, Biocenter Oulu, University of Oulu, Finland

 

Thursday, June 13th, 2013. Gathering in the meeting room 487B, Biocenter Oulu 4th Floor, Aapistie 5A at 8.45 a.m. for demonstrations.

 

9.00-17.00        Demonstrations, 3 groups

 

Demonstration 1. Laser scanning confocal microscopy, Room 421A:

                                      High resolution imaging of living cells in 3D cultures

                                      Bettina Weigelin, Veli-Pekka Ronkainen

 

Demonstration 2. Optical projection tomography (OPT), Room 414A:

         Imaging of whole mouse embryos and tissues

                                      Christoffer Nord, Renata Prunskaite-Hyyryläinen

 

Demonstration 3. Light sheet microscopy, Room 422A:

         Optical sectioning and 3D time lapse imaging of mesoscopic-scale specimens

         Jan Huisken, Lauri Eklund

 

Hours

1st group

2nd group

3rd group

9.00-11.30

Demonstration 1

Demonstration 2

Demonstration 3

11.30-12.00

Lunch

Lunch

Lunch

12.00-14.30

Demonstration 2

Demonstration 3

Demonstration 1

14.30-17.00

Demonstration 3

Demonstration 1

Demonstration 2

 

 

Friday, June 14th, 2013. Meeting Room 487B, Biocenter Oulu 4th Floor, Aapistie 5A / Computer Class KT285

 

9.00-10.30        Computer-based image processing and analysis, Meeting Room 487B

                             Pasi Kankaanpää, Turku Centre for Biotechnology, University of Turku, Finland

 

                             Coffee break

 

10.45-11.30      Novel image analysis tools for biological microscopy images, Meeting Room 487B

                             Mika Kaakinen, Biocenter Oulu, University of Oulu

                            

                             Lunch

 

12.30-16.00      Demonstration 4. Image analysis by BioImageXD, Computer Class KT285

Processing and analysis of captured images and time lapse movies

Pasi Kankaanpää, Mika Kaakinen, Antti Viklund

 

16.00                  Concluding remarks, Veli-Pekka Ronkainen

 

 

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Ritva Saastamoinen, PhD, Docent

Biocenter Oulu Doctoral Programme Coordinator

Adjunct associate professor

 

Biocenter Oulu

PL 5000 (Aapistie 5a)

FIN-90014 University of Oulu, Finland

 

Tel: (+358) 0294 48 6102

E-mail ritva.saastamoinen(at)oulu.fi 

Web pages: http://www.oulu.fi/biocenter/

Fax: (+358) 08 537 6115

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