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From: "Sinikka Eskelinen" <sinikka.eskelinen@oulu.fi>
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Date: Mon, 22 Oct 2007 14:21:36 +0300
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Cc: Sinikka Eskelinen <sinikka.eskelinen@oulu.fi>
Subject: [Ltkhkunta] Lectures in Modern Microscopy
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BIOCENTER <?xml:namespace prefix = st1 ns = "urn:schemas-microsoft-com:office:smarttags" />OULU laboratory course:<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" />
4 th WORK SHOP ON
VISUALISATION OF CELLS AND TISSUES BY LIGHT AND ELECTRON MICROSCOPIC TECHNIQUES AND TOMOGRAPHIC METHODS
Place Auditorium F101 of the Department of Physiology and the Biocenter Oulu Laboratories of the Main Building of the Medical Campus, University of Oulu, Aapistie 5A, P.O.Box 5000, FIN-90014 Oulu, Finland
Time 24th to 26th October, 2007
Credits 1.5 ECTS units for lectures and demonstrations 1 to 4; 0.3 ECTS units for lectures only.
Organisers Biocenter Oulu Core Facilities at the BCO Tissue Imaging Center and the Departments of Medical Biochemistry and Molecular Biology and Pathology, University of Oulu, P.O. Box 5000, FIN-90014 University of Oulu, Finland
Aim To get acquainted with modern imaging technologies and collecting of images of living and fixed cultured cells as well as tissues using video microscope, laser scanning confocal microscope, electron microscope and optical tomography methods
Information Sinikka Eskelinen, Biocenter Oulu, Aapistie 5A, FIN-90014 University of Oulu, Finland, tel. 358-8-537 6101, fax 358-8-537 6115, e-mail sinikka.eskelinen@oulu.fi
URL: http://www.biocenter.oulu.fi/
Registration Registration for lectures recommended, registration for the demonstrations obligatory. Only 16 students are accepted for demonstrations. The application with a short description of the research project should be sent to Sinikka Eskelinen by September 28, 2007. Preference will be given to students whose projects best benefit the topics of the demonstrations given during the course.
PROGRAM
Wednesday, October 24th, 2007, Auditorium F101 of the Department of Physiology of the Medical Campus, Aapistie 7
Chair persons: Sinikka Eskelinen, Raija Sormunen, Aki Manninen
9.00 - 9.15 Opening words by Sinikka Eskelinen
9.15 - 10.15 Live-cell imaging with EosFP and other photoactivatable marker proteins of the GFP family
Jörg Wiedenmann, School of Ocean & Earth Science, University of Southampton National Oceanography Centre, Southampton European Way, Southampton, U.K.
10.15 - 11.15 Imaging Intracellular Signalling
Michael Courtney, Molecular Signalling Laboratory, Department of Neurobiology, A.I. Virtanen Institute, University of Kuopio, Finland
11.15 - 12.30 Lunch
12.30 - 13.30 Optical projection tomography: 3D microscopy of embryos and organs
Jamie Davies, University of Edinburgh, Edinburgh, Scotland, U.K.
13.30 - 14.30 Confocal microscopy of thick specimens
Nathalie Garin, Microscopy, Imaging & Morphology Facility, ISREC, Swiss Federal Institute of Technology in Lausanne, Switzerland
14.30 - 15.00 Coffee
15.00 - 16.00 Electron microscopic 3D imaging
Willie Geerts, Electron Microscopy Group Utrecht, University of Utrecth, The Netherlands
16:00 – 16.15 Concluding remarks by Raija Sormunen
Thursday and Friday, October 25th to 26th , 2007
Gathering in the meeting room 487B of Biocenter Oulu, Aapistie 5A, 4th floor, at 8.45 a.m. on Thursday and Friday morning for demonstrations.
Demonstration groups:
Group 1
1. Kirsi Rilla Ph.D. Biomedicine/Kuopio
2. Ilkka Pietilä M.Sc. Med.biochem.
3. Fumi Suomi M.Sc. Biochemistry
4. Satu Myllymäki B.Sc. Med.biochem
5. Sanna Sillankorva M.Sc. Bioprocessing
Group 2
6. Katja Anttila M.Sc. Biology
7. Elina Kiiskilä B.Sc. Anatomy
8. Mari Aikio M.Sc. Med.biochem
9. Tuomas Huttunen Med.Cand. Med.biochem.
10. Henrika Honkanen M.Sc. Anatomy
Group 3
11. Antti Rivinoja M.Sc. Biochemistry
12. Anniina Vihervaara M.Sc. Turku, Biotech
13. Meng Wang Dipl.Eng Dept.Elect Eng
14. Timo Ruuska M.Sc. Surgery/CRC
15. Matti Kinnunen D.Sc.(Tech) Optoelectronics
Group 4
16. Antti Salo M.Sc. Biochemistry
17. Tiina Kantola M.Sc. Pathology
18. Anne Kunnari M.Sc. Internal med
19. Veli-Pekka Ronkainen M.Sc. Physiology
20. Marjut Tuominen B.Sc. Med biochem
Demonstrations 1 -4:
Thursday
Hours
1st group
2nd group
3rd group
4th group
9 - 12
Confocal microscopy 1
Confocal microscopy 2
Live cell imaging 1&2
Electron microscopy
12 - 13
lunch
lunch
lunch
lunch
13 - 16
Electron microscopy
Confocal microscopy 1
Confocal microscopy 2
Live cell imaging 1&2
Friday
Hours
1st group
2nd group
3rd group
4th group
9 - 12
Live cell imaging 1&2
Electron microscopy
Confocal microscopy 1
Confocal microscopy 2
12 - 13
lunch
lunch
lunch
lunch
13 - 16
Confocal microscopy 2
Live cell imaging 1&2
Electron microscopy
Confocal microscopy 1
Demonstrations:
1. Olympus confocal microscope 1: visualisation of thick specimens, room 423A
Teacher: Nathalie Garin, Lauri Eklund, Mikko Nokkonen
2. Olympus confocal microscope 2 equipped with second laser: photoactivatable marker proteins in living cells, room 422A
Teacher: Jörg Wiedenmann, Aki Manninen, Mikko Nokkonen
3. Live cell imaging 1 using Olympus inverted microscope and Okolab chamber: long term cell culture and wound healing, room 421A
Teacher: Terhi Teräväinen and Riikka Wirkkala
Live cell imaging 2 using Olympus Cell M system: visualisation of cell organelles and cytoskeletal components in living cells, room 419A
Teacher: Sinikka Eskelinen and Linda Rosberg
4. Electron microscopy: 3D tomography of EM images, room 337B (microcomputer class)
Teacher: Willie Geerts, Raija Sormunen
Sinikka Eskelinen, Ph.D.
Coordinator
Biocenter Oulu
University of Oulu
mailing address:
P.O.Box 5000, FIN-90014 University of Oulu
Finland
street address:
Aapistie 5A, FIN-90220 Oulu
Tel. 358-8-5376101
Fax 358-8-5376115
e-mail sinikka.eskelinen@oulu.fi
BIOCENTER URL:http://www.biocenter.oulu.fi
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