Dear All in the first posting some important data was missed unexpectedly.

Please, read the attached pdf-file and especially the BOX if you plan to run any FACS-sorting during corona time.

BR virpi

From: Virpi Glumoff Sent: maanantai 13. huhtikuuta 2020 18.09 To: LTK ltk-staff@oulu.fi; 'fbmm@lists.oulu.fi' fbmm@lists.oulu.fi; 'biolaaket@lists.oulu.fi' biolaaket@lists.oulu.fi; anakoomikot@lists.oulu.fi; 'crc@lists.oulu.fi' crc@lists.oulu.fi; Can_trans@lists.oulu.fi Subject: FACS core RDI service during coronavirus safety regulations

Dear All

Core facilities provide essential services (The University of Oulu Guidelines on Research, Development and Innovation) and therefore also the FACS-core operation continues with the following guidelines: Individual usage of the FACS machines is possible. Max 2 persons at time in the room, keep the 3 m distance. Therefore check all (3) FACS machine reservations in Asimov. There is no guidance for analyses or sorting by the machines. The operators (of the analyzers and the sorter) can run your samples when the sorting protocol is agreed beforehand. All data processing and analysis should be performed offline/home. Virpi can provide data analysis help remotely.

[FACS-sorting service is available according to the new guidelines 1. Inform Virpi by e-mail ( virpi.glumoff@oulu.fi ) about your sorting needs one week before the planned sorting day. * Cell type (species, tissue, primary or cell line) * Number of cell lines * Number of cells to be sorted / cell line * Which fluorophores have been used to label the cells * Number of populations to be sorted (1-4) * Type of collection (6 - 96 well plate, 1.5 - 4 ml tube, 15 ml Falcon) 2. Latest three (3) days before the due date, confirm the exact sorting day and time. 3. If you have to cancel the sorting make it latest by 16:00 on the previous day. 4. The operator will run the samples and pass the sorted samples to you after a phone call. Please, provide your phone number in the e-mail.]mailto:virpi.glumoff@oulu.fi

The researchers who are trained to use FACSAriaIIIu and are able to sort themselves should inform Virpi about their sorting plans and make the reservation in Asimov. Asimov is now open also for FACSAriaIIIu reservations for the trained users. You cannot reserve the operator (Virpi or Sirpa) by Asimov :)

FACS -core facility is open for analysis and sorting under these circumstances by following strict safety regulations given by the University of Oulu https://patio.oulu.fi/en/news/detailed-regulations-working-university-premis...

When you enter the 2nd floor premises: Please remember the rule: one person at a time by the door "Upon entry, each person must log in with his/her personal identification (electric key / ID card) to open the door. * When a door is opened, only one person at a time can enter."

When working in the FACS core room follow these instructions: - use the equipment only if you have made the reservation - max 2 persons at a time in the room - keep at least 3 meters distance to others or wear a face mask (not provided) if working in pairs - wear clean gloves (not provided) - there is Saran wrap (Elmu-kelmu) available to cover the keyboard and mouse during your work. - clean all working surfaces with 70% EtOH or 70% isopropanol before and after the work

Let's work together in a safe way BR virpi

............................................................................. Virpi Glumoff FT, dos., laboratoriopäällikkö Lääketieteellinen tiedekunta, Oulun yliopisto PL 5000 (Aapistie 5A) 90014 Oulun yliopisto

PhD, Adjunct Professor, Laboratory Manager Faculty of Medicine, University of Oulu PO BOX 5000 (Aapistie 5A) FI-90014 UNIVERSITY OF OULU virpi.glumoff@oulu.fimailto:virpi.glumoff@oulu.fi tel: +358 294 485890 .............................................................................