Genetics September 2004

genetics@lists.oulu.fi

3 participants
6 discussions

ABI3730-info
by Helmi Kuittinen 24 Nov '04

24 Nov '04

Hello DNA analyzers, Great that so many attended the 3730 meeting on Tuesday. Those that did not participate in the practical demonstrations, please do not hesitate to ask Johanna Leppälä, Laura Kvist, Sami Sivula or Marjut Kreivi to help you in starting to work with the new machine. You can always also contact Jussi Vanhatalo from Applied Biosystems (09-69379427). There are printed guides for the software available in room PT331. Tutorials for GeneMapper and SeqScape and a guide for sequencing chemistry are available only as CDs. I have downloaded these CDs on the server BIOL4 and you can copy them onto your PC. The two training CDs are quite big and you can also run them directly on CD (CDs are in PT331). When you have samples run in the 3730, the idea is that the machine is told to send your data to the server (BIOL4) into your personal folder. You can then transfer or copy the data from the server to your own computer, where you analyze it with the appropriate software. You will also be able put the sample information in a file to a shared folder (shared/future_runs/36cm_aray or /50cm_array) in the server. Hannele will use that file for the run and then she will move it to the "processed runs" folder. There will be template excell files and instructions available under the "Shared" folder for making these sample informations files. In this way the one PC in PT331 can only be used for running the machine. I have also put guides and training programs (3730 DNA Analyzeres User Guide, 3730 Sequencing chemistry guide, GeneMapper 3.5 training Seqscape 1.1.1 training) under a folder called "Shared/guides/". To get an access to the server BIOL4, you need to write an e-mail to Kai Niemelä, and tell him your name, domain name and whether you are a student or a researcher (graduate students are reseachers). If you want to keep a copy of your data in that server it is possible for some time, we will just need to follow how fast the hard disc will get filled. The software that is used for analyzing the data (SeqA, GeneMapper, and Seqscape) can be donwloaded to your own PC. Seqscape and GeneMapper work only in quite efficient PCs, so pay attention to the requirements. Seqscape and SeqA work with Windows 2000 and XP. The current version of Genemapper only works with Windows 2000, the new (will be available in December) works with XP too. There are three registration codes for the programs and thee simultaneous users are allowed. If you want to download a program, please contact me or Johanna Leppälä. I have attached the special offer for the first order ("kertatilaus laitehankinnan yhteydessä") that I got from Applied Biosystems. I will receive prices for the reagent agreement for new consumables later, maybe not before next Tuesday. I will forward them as soon as I get them. The preliminary information was that in the reagent agreement we will have 35% reduction for plasticware and at least 10% for other things (buffer, polymer, size standards, capillaries); for oligos there was no info. The existing reagent agreement is of course valid and it already includes e.g. the Big Dye kits. The reference price lists of Genomic assays and custom oligos, and Genetic analysis reagents and application kits are available in PT331 and if needed I can send them electronically. I suggest that you compare the regular agreement and the special offer prices, and of course prices from other suppliers, and let me know if you want to order something with the special offer prices. We hope that we will be able to send the order soon, lets aim for Thursday next week. We make a joint order and Sisko will divide the bill for each group accordingly. There are some consumable that need not to be ordered from Applied, e.g. plates (check!). Of the dye set G5, PET, NED, VIC and the size standard LIZ are only produced by them, and with a licence by some other company. At least TAG Copenhagen has a cheaper dye that can be used instead of VIC. We think that it is best that every user/group buys their formamide, because for sequencing there is also the option to put the samples in water, and the sample volume is now much higher (10-20ul). The costs of polymer, buffer, septas etc. will be shared as before, based on the number of samples run by each group. Last, I have booked a computer class (TH118) for the next training session, for 27.-29.9. 8-16. There will be more information in September. I hope these days suit many of you. With regards, Helmi Helmi Kuittinen Oulun yliopisto University of Oulu Biologian laitos Department of Biology PL 3000 P.O.Box 3000, 90014 University of Oulu 90014 Oulun yliopisto Finland email: Helmi.Kuittinen(a)oulu.fi tel. 358-(0)8-553 1803 fax 358-(0)8-553 1061

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Lattiat
by Soile Finne 29 Sep '04

29 Sep '04

Heippa! Muistakaapa ilmoittaa siivojallemme Sarille, milloin teille sopisi työhuoneiden lattioiden pesu ja vahaus 3.kerroksessa!! T.Soile

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(no subject)
by Soile Finne 23 Sep '04

23 Sep '04

Hellurei! Siivojamme aloittaa tänään lattioiden pesun ja vahauksen 3.kerroksessa. Työ tapahtuu aina puolen päivän maissa ja kestää n. tunnista puoleentoista. Alustavasti on sovittu, että tänään pestään sekvensointilabra. Pe 24.9 PT 309 ja Ma 27.9. olisi vuorossa PT 325 eli huone, jossa elektroforeesit ajetaan. Hänen kanssaan voi lopuista huoneista sopia henk.kohtaisesti. T.Soile

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genetiikan seminaarit (genetics seminars)
by Helmi Kuittinen 14 Sep '04

14 Sep '04

hei kaikki, genetiikan seminaarit jatkuvat taas torstaisin klo 12.15 (PT302) biologian torstaiseminaarien kanssa vuorottain, vältämme myös päällekkäisyyttä genetiikan aiheisten tutkimussunnitelmaseminaarien kanssa. Tutkijoilla, jatko-opiskelijoilla, pitkälle edenneillä perustutkinto-opiskelijoilla ja vierailijoilla on mahdollisuus pitää seminaari geneettisestä tai sitä sivuavasta aiheesta. Tilaisuus on vapaamuotoinen eli myös esim. keskeneräisiä tutkimuksia tai suunnitelmia voi esittää. Tämän puitteissa voi myös keskustella mielenkiintoisesta artikkelista tai kertoa muille kongressimatkan uutisia. Seminaarissa käyminen korvaa osallistumisen tutkimussuunnitelmaseminaareihin, jos niitä ei ole ollut riittävästi tarjolla. Ilmoittautukaa esitelmöimään (tai paljastakaa työtoverinne jonka seminaarin haluaisitte kuulla) niin etsitään sopiva ajankohta. Sarjan aloittaa torstaina 16. syyskuuta (PT302, 12.15) Pekka Pamilon vieras, Tom Wenseleers (Oulun yliopisto/University of Leuven) otsikolla: "Are workers in highly eusocial insects altruistic or oppressed?". Terveisin, Helmi Kuittinen ______________________________________________________________________ Genetis seminar series starts again on Thursdays at 12.15, nonoverlapping with Biology thursday seminars. Visitors, researchers and graduate and master´s students can present their research informally, also congress reports and discussion of recent scientific papers can be placed here. Please let me know if you want to give a seminar. The series will be started this Thursday with Pekka Pamilo´s guest Tom Wenseleers (University of Oulu/University of Leuven), who will talk about "Are workers in highly eusocial insects altruistic or oppressed?" (PT302, September 16, at 12.15-13.00) With regards, Helmi Kuittinen Oulun yliopisto University of Oulu Biologian laitos Department of Biology PL 3000 P.O.Box 3000, 90014 University of Oulu 90014 Oulun yliopisto Finland email: Helmi.Kuittinen(a)oulu.fi tel. 358-(0)8-553 1803 fax 358-(0)8-553 1061

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[Evol-ekol] Conference: 38th Population Genetics Group Meeting (fwd)
by Minna Ruokonen 10 Sep '04

10 Sep '04

The 38th annual meeting of the Population Genetics Group will be held at the University of Reading, hosted by the School of Animal and Microbial Sciences. All registration is web-based and details can be found at http://www.rubic.rdg.ac.uk/~pgg ****** PopGroup is an informal meeting which brings together scientists working in population genetics and evolutionary biology, mainly, but by no means exclusively, from the United Kingdom and other European countries. All areas of evolutionary biology are covered. See http://www.lifesci.sussex.ac.uk/CSE/popgroup/programme.pdf for the programme of the previous meeting. This year there will be three plenary talks, given by Rasmus Nielsen, Steve Oliver, and Sara Via. There will be three parallel sessions at other times and a poster session. Talks will be allocated on a first-come-first-served basis. The conference will start on the evening of Tuesday December 14th, with the first talk at 9am on the Wednesday, and run through to lunchtime on Friday December 17th. Reading lies a half-hour train journey on the line between London and Oxford. It is also about an hour's coach journey from Heathrow Airport. All the conference facilities are on campus and within easy walking distance of each other. Reading is easily accessible by rail, road and air (see travel information and maps). -- Mark A. Beaumont, School of Animal and Microbial Sciences, University of Reading, Whiteknights, P.O. Box 228, Reading RG6 6AJ, UK Tel 0118 378 7707 Fax 0118 931 0180 Email: m.a.beaumont(a)reading.ac.uk WWW: http://www.rubic.rdg.ac.uk/~mab/ _______________________________________________ Evol-ekol mailing list Evol-ekol(a)lists.jyu.fi http://lists.jyu.fi/mailman/listinfo/evol-ekol

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(no subject)
by Soile Finne 09 Sep '04

09 Sep '04

Hei! Marika Kuusela LabNetistä tulee esittelemään tuotteita tänään kahvihuoneeseen klo. 13.00!! Pullatarjoilu! T. Soile

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Genetics September 2004