hei,
finally I got the information about the additions to the reagent agreement EW031104 (see attachment 2). In summary, we will get reductions that are almost as good as in the special offer VP040821 until the end of the year (but note that the list prices will have their yearly increment in October, see attachment 1). However, we did not get any information at all about the prices next year. Earlier Virpi Parviainen promised that we would get 35% reduction for plasticware and at least 10% for everything else, now she only says that it will depend on the negociation at the end of the year.
If you want to order oligos this week, we will make a joint order under VP040821 (bring me the information indicated below e.g. by email), but you can as well do it individually referring to the reagent agreement (EW031104) because the prices are the same as in the special offer VP040821. The oligos can be ordered with the following instructions (remember to refer to EW031104).
Write an email to finland.order@eur.appliedbiosystems.com with the following information. a) tuotteen nimi ja tuotenumero (Readypure ABI Prism Primer, P/N 450007, maksimipituus 30 bp)(name and code of the product, max length of the primer=30bp)) b) primerin nimi (Cox-2) (name of the primer) c) primerin sekvenssi (5'acgt......cgta3') (sequence) d) haluttu leima (6-FAM, TET, VIC, HEX, NED, PET) (label)
A few things about consumables and reagents: We have figured out that it would be fare and simple if all groups/researcher would buy plastic plates for their sequencing and fragment analysis samples to be run in ABI3730. The machine needs a barcode on the plate. Septas will be provided by Hannele Parkkinen, she will cover the plates with septas when she prepares them for a run. If you keep your samples e.g. in a fridge or a freezer before bringing them to PT331, you should cover them with plastic or folio.
Those researchers who only have a few samples to be run can share a plate in the same way as before (the first one should bring the plate).
And, please remember that you should not write on top of the plate, ink may damage capillaries. You are allowed to write on sides.
In summary: plates and formamide are provided by the groups; septas, polymer, buffer are ordered by Hannele and the costs are shared by the users. Capillary arrays need to be replaced once in a while and the costs will also be shared by the users. I have calculated that the cost of capillaries would be about 13 cnt/sample (including ALV), please take this into account in your budget.
Please let me know if you want to order something, at last tomorrow at 4 pm.
With regards, Helmi
Helmi Kuittinen
Oulun yliopisto University of Oulu Biologian laitos Department of Biology PL 3000 P.O.Box 3000, 90014 University of Oulu 90014 Oulun yliopisto Finland
email: Helmi.Kuittinen@oulu.fi tel. 358-(0)8-553 1803 fax 358-(0)8-553 1061