23 Jan
2004
23 Jan
'04
10:20 a.m.
Hei!
I´m doing my master´s theses in the Bioprocess Engineering Department at the University of Oulu. I want to repair a frameshift mutation in an E. coli gene and it has been reported that the frameshift can be repaired by inserting 2 basepairs or deleting 1 bp in a 10bp region preceding the frameshift site. Does anybody know how to get 2bp in there? I thought about homologous recombination, phages,..? The problem is that I should not put a resistence marker in (-> screening??). Are there any integration vectors I could use? If sombody has experience with that, please contact me! Thanks in advance, Christina