Hi,

Finnzymes has given us a small amount of Sybr Safe dye, which can be used instead of ethidium bromide on agarose gels. Sybr Safe should be less toxic compared to EtBr, but we are still trying to find out what kind of precautions and waste disposal are necessary (an email from Finnzymes below).

Soile tried Sybr Safe and it worked nicely, also photos were OK with the camera we have now (no specific instruments or even filters necessary). If you want to try it yourself, ask Soile.

The price for 400 microliters is 67 eur (November 2005), 1 microliter of dye / 10 ml of gel solution is needed.

Minna

---------- Forwarded message ---------- Date: Wed, 29 Mar 2006 09:16:59 +0300 From: Katja Mäntylä katja.mantyla@finnzymes.fi To: 'Minna Ruokonen' mruokone@sun3.oulu.fi Subject: FW: Sybr safe -jätteenkäsittely Euroopassa

Hei Minna,

Alla vielä Invitrogenilta saamani vastaus koskien Sybr Safe geelivärijätteen käsittelyä Euroopassa. Vastaus on melko ylimalkainen johtuen siitä että paikallisten viranomaisten ohjeet vaihtelevat maakohtaisesti.

Toivottavasti tästä on kuitenkin jotain apua!

Ystävällisin terveisin, Katja ************************** Katja Mäntylä, M.Sc. Product Manager

Finnzymes Oy Keilaranta 16 A 02150 Espoo Finland Tel. +358 9 5841 2246 Fax +358 9 5841 2200 Katja.mantyla@finnzymes.fi

-----Original Message----- From: Yamaoka, Larry [mailto:larry.yamaoka@invitrogen.com] Sent: 22. helmikuuta 2006 1:31 To: Finnzymes Oy (Katya); !EUG-Sybrsafe Subject: RE: Sybr safe waste handling in Europe

Dear Katja: We do not have any specific suggestions (for Europe) for disposal of SYBR Safe DNA gel stain. Because local rules may differ from Federal (USA) guidelines, the suggestion from our environmental safety office is: -characterize the material you want to dispose -go to the local authority that has jurisdiction over route(s) of disposal (e.g., landfill, wastewater, etc.) -show them what you know about your waste (i.e., the information provided in the SYBR Safe product manual) -ask them if they approve the disposal. Please contact me if you have other questions or require additional information.

Sincerely, Larry Yamaoka, Ph.D. Invitrogen Corporation Molecular Probes Detection Technologies Technical Support Scientist Phone: 541 335 0353 Fax: 541 335 0238 larry.yamaoka@invitrogen.com

_____

From: Katja Mäntylä [mailto:katja.mantyla@finnzymes.fi] Sent: Tuesday, February 21, 2006 4:27 AM To: !EUG-Sybrsafe Subject: Sybr safe waste handling in Europe Dear Receiver, Have you got any European guidelines for the waste handling of Sybr safe DNA gel stain? Thanks and kind regards, Katja ************************** Katja Mäntylä, M.Sc. Product Manager

Finnzymes Oy Keilaranta 16 A 02150 Espoo Finland Tel. +358 9 5841 2246 Fax +358 9 5841 2200 Katja.mantyla@finnzymes.fi