Subject: Doctoral (PhD) student position in Reproductive Toxicology at Karolinska Institutet

Doctoral (PhD) student position in Reproductive Toxicology at Karolinska Institutet We are recruiting a doctoral (PhD) student. For more information regarding doctoral education at Karolinska Institutet (KI), see: http://ki.se/en/phd/about-doctoral-education-at-ki A doctoral student is admitted to third-cycle/doctoral education. A doctoral education consists of supervised research, combined with courses and other educational activities. An individual study plan is established for each doctoral student. For a doctoral degree, the equivalent of four years of full-time doctoral education is required. Research group The doctoral education project will be carried out in Associate Professor Pauliina Damdimopoulou's research laboratory at the Division of Obstetrics and Gynecology at the Department of Clinical Science, Intervention and Technology of Karolinska Institutet. Research and education at the Division of Obstetrics and Gynecology are focused on the many aspects of women's reproductive health as well as fetal physiology and stem cell biology. Damdimopoulou laboratory is located at the Campus Flemingsberg of Karolinska Institutet and focuses on human ovarian biology as well as effects of toxic chemicals on fertility. The doctoral student project and the duties of the doctoral student The doctoral education project will be carried out within the Horizon2020 project FREIA (http://www.freiaproject.eu/ ), which rises from a concern that environmental factors can affect a woman's chances of becoming pregnant. Hence, the ambition of FREIA is to develop novel identification and screening approaches for endocrine disrupting chemicals (EDCs) that target female fertility. The aim of this doctoral education project is to study the effects of EDCs on human ovarian follicle growth in vitro and identify associated adverse outcome pathways. Cross-species validation will be carried out by comparing the findings to rat studies carried out in FREIA. In addition, ovarian transcriptomics datasets will be used to understand possible age-dependent differences in chemical toxicity. The work will require visits to laboratories of co-supervisors in Finland, Denmark and Estonia. The duties of the doctoral student include cell and tissue culture, gene expression analyses and histological analyses. In addition, the student will assist in collection and analyses of rat ovaries and oocytes. The project also involves analyses of RNA-seq datasets. Techniques utilized will include in vitro culture of human ovarian tissue samples, isolation and culture of primary ovarian cells, immunostaining, fluorescent microscopy and histomorphometric analyses, as well as bioinformatics analyses in R. More information and application at https://ki.mynetworkglobal.com/en/what:job/jobID:273819/?fbclid=IwAR2OXCXXao... Last application date is June 20, 2019 11:59 PM CET.