Hi,

Registration to the Method Course on Immunohistochemistry (April 24-25) is now open until April 17. The course info is available also in the HBS-DP web pages: see our calendar of events at http://www.oulu.fi/uniogs/dp/hbsdp/calendar-of-events. Herehttp://www.oulu.fi/sites/default/files/content/Immunohisto%20Course%20Oulu%202018.pdf is the direct link to the programme pdf.

Best wishes, Ritva ***********

Method Course on Immunohistochemistry (1 ECTS), April 24-25, 2018

Venue:

Biocenter Oulu histology lab 479B, Kontinkangas, Aapistie 5A, 90220 Oulu

Teacher:

Lotta Leveelahti, Histolab Products Oy

Organizers:

Health and Biosciences Doctoral Programme, Biocenter Oulu Transgenic Mouse Core Facility, and Histolab Products Oy

Credits:

1.0 ECTS for field-specific studies in doctoral training

Enquiries:

Dr. Reetta Vuolteenaho, reetta.vuolteenaho(at)oulu.fi

Registration:

The course is limited to about 12 participants selected by application. Priority is given to doctoral students. Online registration/application, deadline April 17. All applicants will be notified on April 18.

This 2-day practical course includes short lectures on each step of the immunohistochemistry (IHC) protocol. Additionally, you get hands-on experience about the micropolymer detection method with human tissue samples. Micropolymer detection enables superior sensitivity and reduced background staining compared to the traditional avidin-biotin-based detection. In the course, we will this time perform simultaneous chromogenic multiplex staining on a paraffin section. Is it as easy as doing a single stain? Come and see!

The course participants will do single stainings using their own mouse tissue sections (3-4 slides) and their own primary antibody which has been tested for immunohistochemistry. The human tissue samples, secondary antibodies and reagents for double stainings will be provided by the organizers.

Apply online: https://www.webropolsurveys.com/S/24D17892DA717B7B.par

Course programme

Day 1 (9.00-15.00) 9.00-10.00 Intro IHC + Pre-treatments + Blocking 10.00-10.15 Lab: Deparaffinisation and start pre-treatments 10.15 - 10.30 Break 10.30-11.00 Antibodies 11.00-11.15 Lab: Slides into TBS 11.15 - 11.45 Detection 11.45-12.00 Lab: Background blocking and application of antibody 12.00-13.00 Lunch break 13.00 - 13.15 Lab: Application of Probe 13.15 - 13.30 Chromogen + counterstaining + Mounting 13.30 - 13.45 Lab: Application of Polymer 13.45 - 14.15 Trouble shooting + Wrap Up 14.15 - 15.00 Lab: Application of Chromogen + Counter staining + Mounting + Check results

Day 2 (9.00-12.45) 09.00 -09.20 Lab: Deparaffinisation and start pre-treatments 09.20 - 10.00 Multiplex IHC 10.00 -10.05 Lab: Slides into TBS 10.05-10.15 Break 10.15-10.30 Lab: Background blocking and application of antibody cocktail 10.30 - 11.00 Trouble shooting 11.00 - 11.10 Lab: application of polymer cocktail 11.10 - 11.40 Lunch break 11.40 - 12.00 Lab: Application of Chromogen 12.00 - 12.15 Questions and discussions 12.15 - 12.45 Counterstaining+ Mounting + check results

**************************************************************** Ritva Saastamoinen, PhD, Docent Coordinator, Adjunct associate professor Health and Biosciences Doctoral Programme (HBS-DP)

Biocenter Ouluhttp://www.oulu.fi/biocenter/ and University of Oulu Graduate Schoolhttp://www.oulu.fi/uniogs/ P.O.Box 5000, FIN-90014 University of Oulu, Finland (Visiting address: Aapistie 5A, Room 473B)

ritva.saastamoinen(at)oulu.fi Tel: +358 294 48 6102, mobile +358 50 526 7164 http://www.oulu.fi/uniogs/dp/hbsdp ****************************************************************