Biology Thursday seminar 29th Oct: Dr. Marko Prous: Nanopore sequencing to replace Sanger
Dear colleagues,
this week's Biology Thursday Seminar (29th Oct, 12:15, zoom link below) will be given by Dr. Marko Prous, University of Tartu, Dept. of Zoology, on
"Nanopore sequencing to replace Sanger"
Abstract
High-throughput sequencing methods (Illumina, PacBio, Oxford Nanopore) have become routine in many laboratories. Nevertheless, low-throughput Sanger sequencing is still widely used in smaller scale projects, but also in large-scale DNA barcoding efforts of large number of specimens based on a single or a few genes. It will be argued that Sanger sequencing can be replaced with Nanopore sequencing as long as tens of specimens and several genes or hundreds of specimens and one gene are sequenced in one experiment. With Nanopore, fragments of any length (DNA or RNA) can be sequenced and intraindividual variants (due to heterozygosity, heteroplasmy, or paralogy) can be resolved. Nanopore has a reputation of low sequencing accuracy, but this has significantly improved and is now mature enough to get consensus sequences with very similar accuracy to other technologies (likely even better compared to Sanger).
More on Marko's work at: http://lepo.it.da.ut.ee/~mprous/
See you on Thursday!
Heikki
Heikki Helanterä is inviting you to a scheduled Zoom meeting.
Topic: Biology Thursday seminar Time: This is a recurring meeting Meet anytime
Join Zoom Meeting https://oulu.zoom.us/j/62917763539?pwd=WENZRy9Bb3NlSzlyeVlVamJ4RHR5Zz09
Meeting ID: 629 1776 3539 Passcode: 166151 One tap mobile +496938079883,,62917763539# Germany +496950502596,,62917763539# Germany
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=============================================== Heikki Helanterä Associate Professor (tenure track) Ekologian ja genetiikan tutkimusyksikkö / Ecology and Genetics Research Unit Oulun Yliopisto / University of Oulu / Finland heikki.helantera@oulu.fimailto:heikki.helantera@oulu.fi +358-40-7507 334
Hi all,
This is just a reminder of the very interesting Thursday seminar talk given today at 12.15 by Marko Prous.
Please join the seminar! The link is below.
Marko
From: biolaitos-bounces@lists.oulu.fi biolaitos-bounces@lists.oulu.fi On Behalf Of Heikki Helanterä Sent: Monday, October 26, 2020 11:34 AM To: biology-students@lists.oulu.fi; biolaitos@lists.oulu.fi; bioposti@lists.oulu.fi Subject: [Biolaitos] Biology Thursday seminar 29th Oct: Dr. Marko Prous: Nanopore sequencing to replace Sanger
Dear colleagues,
this week's Biology Thursday Seminar (29th Oct, 12:15, zoom link below) will be given by Dr. Marko Prous, University of Tartu, Dept. of Zoology, on "Nanopore sequencing to replace Sanger"
Abstract
High-throughput sequencing methods (Illumina, PacBio, Oxford Nanopore) have become routine in many laboratories. Nevertheless, low-throughput Sanger sequencing is still widely used in smaller scale projects, but also in large-scale DNA barcoding efforts of large number of specimens based on a single or a few genes. It will be argued that Sanger sequencing can be replaced with Nanopore sequencing as long as tens of specimens and several genes or hundreds of specimens and one gene are sequenced in one experiment. With Nanopore, fragments of any length (DNA or RNA) can be sequenced and intraindividual variants (due to heterozygosity, heteroplasmy, or paralogy) can be resolved. Nanopore has a reputation of low sequencing accuracy, but this has significantly improved and is now mature enough to get consensus sequences with very similar accuracy to other technologies (likely even better compared to Sanger).
More on Marko's work at: http://lepo.it.da.ut.ee/~mprous/
See you on Thursday!
Heikki
Heikki Helanterä is inviting you to a scheduled Zoom meeting.
Topic: Biology Thursday seminar Time: This is a recurring meeting Meet anytime
Join Zoom Meeting https://oulu.zoom.us/j/62917763539?pwd=WENZRy9Bb3NlSzlyeVlVamJ4RHR5Zz09
Meeting ID: 629 1776 3539 Passcode: 166151 One tap mobile +496938079883,,62917763539# Germany +496950502596,,62917763539# Germany
Dial by your location +49 69 3807 9883 Germany +49 695 050 2596 Germany +49 69 7104 9922 Germany +49 30 5679 5800 Germany Meeting ID: 629 1776 3539 Find your local number: https://oulu.zoom.us/u/clPLBw2vz
Join by SIP 62917763539@109.105.112.236mailto:62917763539@109.105.112.236 62917763539@109.105.112.235mailto:62917763539@109.105.112.235
Join by H.323 109.105.112.236 109.105.112.235 Meeting ID: 629 1776 3539 Passcode: 166151
=============================================== Heikki Helanterä Associate Professor (tenure track) Ekologian ja genetiikan tutkimusyksikkö / Ecology and Genetics Research Unit Oulun Yliopisto / University of Oulu / Finland heikki.helantera@oulu.fimailto:heikki.helantera@oulu.fi +358-40-7507 334
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Heikki Helanterä -
Marko Mutanen