Biocenter Oulu is organizing the 4th workshop on
VISUALISATION OF CELLS AND TISSUES BY LIGHT AND ELECTRON MICROSCOPIC TECHNIQUES AND TOMOGRAPHIC METHODS
<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" />
Place Auditorium F101 of the Department of Physiology and the Biocenter Oulu Laboratories of the Main Building of the Medical Campus, University of Oulu, Aapistie 5A, P.O.Box 5000, FIN-90014 Oulu, Finland
Time 24th to 26th October, 2007
Credits 1.5 ECTS units for lectures and demonstrations 1 to 4; 0.3 ECTS units for lectures only.
Organisers Biocenter Oulu Core Facilities at the BCO Tissue Imaging Center and the Departments of Medical Biochemistry and Molecular Biology and Pathology, University of Oulu, P.O. Box 5000, FIN-90014 University of Oulu, Finland
Aim To get acquainted with modern imaging technologies and collecting of images of living and fixed cultured cells as well as tissues using video microscope, laser scanning confocal microscope, electron microscope and optical tomography methods
Information Sinikka Eskelinen, Biocenter Oulu, Aapistie 5A, FIN-90014 University of Oulu, Finland, tel. 358-8-537 6101, fax 358-8-537 6115, e-mail sinikka.eskelinen@oulu.fi
URL: http://www.biocenter.oulu.fi/
Registration Registration for lectures recommended, registration for the demonstrations obligatory. Only 16 students are accepted for demonstrations. The application with a short description of the research project should be sent to Sinikka Eskelinen by September 28, 2007. Preference will be given to students whose projects best benefit the topics of the demonstrations given during the course.
PROGRAM
Wednesday, October 24th, 2007, Auditorium F101 of the Department of Physiology of the Medical Campus, Aapistie 7
Chair persons: Sinikka Eskelinen, Raija Sormunen, Aki Manninen
9.00 - 9.15 Opening words by Sinikka Eskelinen
9.15 - 9.45 Live-cell imaging with EosFP and other photoactivatable marker proteins of the GFP family
Jörg Wiedenmann, School of Ocean & Earth Science, <?xml:namespace prefix = st1 ns = "urn:schemas-microsoft-com:office:smarttags" />University of Southampton National Oceanography Centre, Southampton European Way, SOUTHAMPTON, U.K.
9.45 10.15 Live cell imaging/News from Olympus
Aki Manninen, Biocenter Oulu/Representative from Olympus
10.15 - 11.15 Imaging Intracellular Signalling
Michael Courtney, Molecular Signalling Laboratory, Department of Neurobiology, A.I. Virtanen Institute, University of Kuopio, Finland
11.15 - 12.30 Lunch
12.30 - 13.30 Optical projection tomography: 3D microscopy of embryos and organs
Jamie Davies, University of Edinburgh, Edinburgh, Scotland, U.K.
13.30 - 14.30 Confocal microscopy of thick specimens
Nathalie Garin, Microscopy, Imaging & Morphology Facility, ISREC, Swiss Federal Institute of Technology in Lausanne, Switzerland
14.30 - 15.00 Coffee
15.00 - 16.00 Electron microscopic 3D imaging
Willie Geerts, Electron Microscopy Group Utrecht, University of Utrecth, The Netherlands
16:00 16.15 Concluding remarks by Raija Sormunen
Thursday and Friday, October 25th to 26th , 2007
There will be four groups of four students participating in the demonstrations, two demonstrations per day.
Demonstrations:
- Olympus confocal microscope 1: visualisation
of thick specimens, room 423A
Teacher: Nathalie Garin, Aki Manninen
- Olympus confocal microscope 2 equipped with
second laser: photoactivatable marker proteins in living cells, room 422A
Teacher: Jörg Wiedenmann, Aki Manninen
- Live cell imaging 1 using Olympus inverted
microscope and Okolab chamber: long term cell culture and wound healing, room 421A
Teacher: Terhi Teräväinen and Riikka Wirkkala
Live cell imaging 2 using Olympus Cell M system: visualisation of cell organelles and cytoskeletal components in living cells, room 419A Teacher: Sinikka Eskelinen and Linda Rosberg
- Electron microscopy: 3D tomography of EM
images, room 337B (microcomputer class)
Teacher: Willie Geerts
Sinikka Eskelinen, Ph.D. Coordinator Biocenter Oulu University of Oulu mailing address: P.O.Box 5000, FIN-90014 University of Oulu Finland street address: Aapistie 5A, FIN-90220 Oulu Tel. 358-8-5376101 Fax 358-8-5376115 e-mail mailto:sinikka.eskelinen@oulu.fisinikka.eskelinen@oulu.fi BIOCENTER http://www.biocenter.oulu.fiURL:http://www.biocenter.oulu.fi
Ltkhkunta mailing list Ltkhkunta@lists.oulu.fi http://lists.oulu.fi/mailman/listinfo/ltkhkunta
Tiina Hurskainen, Ph.D. Laboratory Manager Clinical Research Center Oulu University Hospital Aapistie 5A 90220 Oulu Finland tel: +358-8-5376302 or +358-40-7040270 e-mail: tiina.hurskainen@oulu.fi