Subject: [Ltkhkunta] Basic course on light microscopy, June 11-13, Oulu
BIOCENTER OULU, Light Microscopy Core Facility Course BASIC COURSE ON LIGHT MICROSCOPY
Place Auditorium P117 and BCO Light Microscopy Core Facility Laboratories, the Main Building of the Medical Campus, University of Oulu, Aapistie 5A, 90220 Oulu, Finland Time 11th to 13th June, 2014 Credits 1 ETCS Organisers Biocenter Oulu Doctoral Programme and Biocenter Oulu Tissue Imaging Center, University of Oulu, Aapistie 5A, P.O.Box 5000, FI-90014 University of Oulu, Finland, URL: http://www.oulu.fi/biocenter/tic Aim To get acquainted with light and fluorescence imaging methods of living and fixed microscopic specimens. Learn how to apply suitable techniques for analysis of images and time lapse movies. Introduction includes basic microscopy, laser scanning and spinning disc confocal microscopy and light sheet fluorescence microscopy.
Information Veli-Pekka Ronkainen, Biocenter Oulu, Aapistie 5A, P.O.Box 5000, FI-90014, University of Oulu, Finland, tel. +358 294 486114, e-mail: veli-pekka.ronkainen(at)oulu.fimailto:veli-pekka.ronkainen@oulu.fi
The course consists of lectures, independent learning, microscopy demonstrations and computer-based image analysis.
Registration Lectures are open for all without registration. Registration for the demonstrations is obligatory and preference will be given to Ph.D. students and researchers whose project benefits the topics carried out during the course. Due to space limitations only 18 participants can be accepted. The application with a short description of the applicant's project should be sent by email to Veli-Pekka Ronkainen (veli-pekka.ronkainen(at)oulu.fimailto:veli-pekka.ronkainen@oulu.fi) by 23th May, 2014.
PROGRAMME
Wednesday, June 11th, 2014. Auditorium P117 (Pathology)
Lectures:
9.00-9.05 Opening words and introduction
Sinikka Eskelinen, Biocenter Oulu
9.05-10.00 Basics in light/fluorescence microscopy Sinikka Eskelinen
10.00-10.15 Coffee break
10.15-11.00 Confocal microscopy techniques
Veli-Pekka Ronkainen, Biocenter Oulu
11.00-11.15 Break
11.15-12.15 Challenges in live cell imaging
Group work in 2-3 person groups
Veli-Pekka Ronkainen, Janne Capra and Fumi Suomi, Biocenter Oulu
12.15-13.15 Lunch
13.15-14.00 Principles of light sheet fluorescence microscopy
Veli-Pekka Ronkainen
14.00-14.15 Break
14.15-15.15 Examples of practical applications utilizing light microscopy
Invited speakers from local research groups, short talks
15.15-16.00 Introduction to demonstrations
Veli-Pekka Ronkainen
Thursday, June 12th, 2014. Gathering in the meeting room 487B, Biocenter Oulu 4th Floor, Aapistie 5A at 8.45 a.m. for demonstrations.
9.00-16.00 Demonstrations, 3 groups
Demo 1. Laser scanning confocal microscopy, Room 421A
Imaging of hypoxic living cells and 3D imaging of thick specimens
Veli-Pekka Ronkainen, Biocenter Oulu
Demo 2. Spinning disc confocal microscopy, Room 422A
Live cell fluorescence and time lapse overnight imaging, molecular dynamics (FRAP)
Fumi Suomi, Biocenter Oulu
Demo 3. Imaging colocalization with a confocal microscope, Room 423A
Imaging and visualization of colocalization in multi-labelled samples
Janne Capra, Biocenter Oulu
Hours
1st group
2nd group
3rd group
9.00-11.00
Demonstration 1
Demonstration 2
Demonstration 3
11.00-12.00
Lunch
Lunch
Lunch
12.00-14.00
Demonstration 2
Demonstration 3
Demonstration 1
14.00-16.00
Demonstration 3
Demonstration 1
Demonstration 2
Friday, June 13th, 2014. Meeting Room 487B, Biocenter Oulu 4th Floor / Computer Class KT285
9.00-10.00 Computer-based image processing and analysis, Meeting Room 487B
Basic introduction to image processing
Neslihan Bayramoglu, Center for machine vision research, University of Oulu
Segmentation of fluorescently labeled cells in 3D matrix,
Saad Ullah Akram, Center for machine vision research, University of Oulu
10.00-10.15 Coffee break
10.15-11.00 Computer-based processing and analysis of captured images and time lapse movies, Computer Class KT285
Veli-Pekka Ronkainen, Antti Viklund, Fumi Suomi
11.00-12.00 Lunch
12.00-14.00 Computer-based processing and analysis of captured images and time lapse movies continues, Computer Class KT285
Veli-Pekka Ronkainen, Antti Viklund, Fumi Suomi
14.00 Concluding remarks, Veli-Pekka Ronkainen
Course programme available at http://www.oulu.fi/sites/default/files/content/Basic_microscopy_course_June_... . It can be found also via the Biocenter Oulu front pagehttp://www.oulu.fi/biocenter under "Events", BCO Calendar of eventshttp://www.oulu.fi/biocenter/calendar-of-events and the Oulu BioImaging education pagehttp://www.oulu.fi/obi/education.
If you have any questions, please contact Dr. Veli-Pekka Ronkainen, tel. +358 294 486114, e-mail: veli-pekka.ronkainen(at)oulu.fimailto:veli-pekka.ronkainen@oulu.fi.
Kind regards, Ritva
*************************************** Ritva Saastamoinen, PhD, Docent Biocenter Oulu Doctoral Programme Coordinator Adjunct associate professor
Biocenter Oulu PL 5000 (Aapistie 5a) FIN-90014 University of Oulu, Finland
Tel: (+358) 0294 48 6102 ritva.saastamoinen(at)oulu.fi http://www.oulu.fi/biocenter/doctoral-programme ***************************************