Subject: [Ltkhkunta] 6th WORKSHOP on Visualisation of Cells and Tissues by Light Microscopic Techniques and Tomographic Methods, June 12-14, Oulu

Hi everyone,

Biocenter Oulu Doctoral Programme (BCO-DP) and Biocenter Oulu Tissue Imaging Center are organizing a workshop on Visualisation of Cells and Tissues by Light Microscopic Techniques and Tomographic Methods. The course will present lectures to Ph.D. students and all scientists interested in the latest advanced microscopic and mesoscopic imaging methods. Demonstrations are limited to Ph.D. students and researchers whose projects benefit the topics carried out during the course.

Teachers of the course are pioneering experts in the field. Jan Huisken (Max Planck Institute, Dresden, Germany) has been developing SPIM technique and used it very successfully for imaging thick biological specimens, Bettina Weigelin (Radboud University Nijmegen, The Netherlands) has developed methods for tumor intravital- and 3D organotypic imaging, Ulf Ahlgren (University of Umeå, Sweden) is an expert in optical projection tomography methods for developmental biology applications and Pasi Kankaanpää (University of Turku, Finland) is one of the creators of a novel open-source image processing and analysis software BioImageXD.

Please find the detailed programme and more information from below and via this link: http://www.oulu.fi/sites/default/files/content/Advanced_microscopy_course_Ju... .

Kind Regards, Ritva

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6th WORKSHOP on Visualisation of Cells and Tissues by Light Microscopic Techniques and Tomographic Methods

Place Auditorium F202 and BCO Light Microscopy Core Facility Laboratories, the Main Building of the Medical Campus, University of Oulu, Aapistie 5A, 90220 Oulu, Finland Time 12th to 14th June, 2013 Credits 1 ETCS Organisers Biocenter Oulu Doctoral Programme and Biocenter Oulu Tissue Imaging Center, University of Oulu, Aapistie 5A, P.O.Box 5000, FIN-90014 University of Oulu, Finland, URL: http://www.oulu.fi/biocenter/tic Aim To get acquainted with light and fluorescence imaging methods of living and fixed microscopic and mesoscopic scale specimens using laser scanning confocal microscopy, light sheet microscopy and optical projection tomography. Learn how to apply suitable techniques for analysis of microscopic images and time lapse movies.

Information Veli-Pekka Ronkainen, Biocenter Oulu, Aapistie 5A, P.O.Box 5000, FIN-90014, University of Oulu, Finland, tel. +358 294 486114, e-mail: veli-pekka.ronkainen(at)oulu.fimailto:veli-pekka.ronkainen@oulu.fi

The course consists of lectures, independent learning, microscopy demonstrations and computer-based image analysis.

Registration Lectures are open for all without registration. Registration for the demonstrations is obligatory and preference will be given to Ph.D. students and researchers whose project benefits the topics carried out during the course. Due to space limitations only 18 participants can be accepted. The application with a short description of the applicant’s project should be sent by email to Veli-Pekka Ronkainen (veli-pekka.ronkainen(at)oulu.fimailto:veli-pekka.ronkainen@oulu.fi) by 24th May, 2013.

PROGRAMME

Wednesday, June 12th, 2013. Auditorium F202 (Pharmacology), The Main Building of the Medical Campus, Aapistie 5A

Lectures:

9.00-9.05 Opening words and introduction

Sinikka Eskelinen/Veli-Pekka Ronkainen, Biocenter Oulu, University of Oulu, Finland

9.05-10.35 Confocal-, multiphoton- and higher harmonic generation microscopy to visualize microanatomy and dynamic processes in live tissues

Bettina Weigelin, Nijmegen Center for Molecular Life Sciences, Radboud University Nijmegen, The Netherlands

10.35-10.45 Coffee break

10.45-12.15 Optical Projection Tomography imaging of embryos and organs - studies from pancreas development and diabetes disease aetiology

Ulf Ahlgren and Christoffer Nord, Umeå Centre for Molecular Medicine, University of Umeå, Sweden

12.15-13.00 Lunch

13.00-14.30 Principles and applications of light-sheet microscopy

Jan Huisken, Max-Planck-Institute of Molecular Cell Biology and Genetics, Dresden, Germany

14.30-14.45 Coffee break

14.45-15.15 Commercial Light Sheet Z.1 Microscope for biological specimens

Product Manager, Carl Zeiss, Germany

15.15-16.00 Introduction to demonstrations

Veli-Pekka Ronkainen, Biocenter Oulu, University of Oulu, Finland

Thursday, June 13th, 2013. Gathering in the meeting room 487B, Biocenter Oulu 4th Floor, Aapistie 5A at 8.45 a.m. for demonstrations.

9.00-17.00 Demonstrations, 3 groups

Demonstration 1. Laser scanning confocal microscopy, Room 421A:

High resolution imaging of living cells in 3D cultures

Bettina Weigelin, Veli-Pekka Ronkainen

Demonstration 2. Optical projection tomography (OPT), Room 414A:

Imaging of whole mouse embryos and tissues

Christoffer Nord, Renata Prunskaite-Hyyryläinen

Demonstration 3. Light sheet microscopy, Room 422A:

Optical sectioning and 3D time lapse imaging of mesoscopic-scale specimens

Jan Huisken, Lauri Eklund

Hours

1st group

2nd group

3rd group

9.00-11.30

Demonstration 1

Demonstration 2

Demonstration 3

11.30-12.00

Lunch

Lunch

Lunch

12.00-14.30

Demonstration 2

Demonstration 3

Demonstration 1

14.30-17.00

Demonstration 3

Demonstration 1

Demonstration 2

Friday, June 14th, 2013. Meeting Room 487B, Biocenter Oulu 4th Floor, Aapistie 5A / Computer Class KT285

9.00-10.30 Computer-based image processing and analysis, Meeting Room 487B

Pasi Kankaanpää, Turku Centre for Biotechnology, University of Turku, Finland

Coffee break

10.45-11.30 Novel image analysis tools for biological microscopy images, Meeting Room 487B

Mika Kaakinen, Biocenter Oulu, University of Oulu

Lunch

12.30-16.00 Demonstration 4. Image analysis by BioImageXD, Computer Class KT285

Processing and analysis of captured images and time lapse movies

Pasi Kankaanpää, Mika Kaakinen, Antti Viklund

16.00 Concluding remarks, Veli-Pekka Ronkainen

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******************************************* Ritva Saastamoinen, PhD, Docent Biocenter Oulu Doctoral Programme Coordinator Adjunct associate professor

Biocenter Oulu PL 5000 (Aapistie 5a) FIN-90014 University of Oulu, Finland

Tel: (+358) 0294 48 6102 E-mail ritva.saastamoinen(at)oulu.fi Web pages: http://www.oulu.fi/biocenter/ Fax: (+358) 08 537 6115 *******************************************