X-Spam-Checker-Version: SpamAssassin 3.1.5 (2006-08-29) on sun3.oulu.fi X-Spam-Level: X-Spam-Status: No, score=-3.0 required=5.0 tests=ALL_TRUSTED,BAYES_00, HTML_90_100,HTML_MESSAGE,MIME_HTML_MOSTLY,TW_KH,TW_TK autolearn=ham version=3.1.5 X-Virus-Scanned: amavisd-new at lists.oulu.fi X-Original-To: ltkhkunta@lists.oulu.fi Delivered-To: ltkhkunta@lists.oulu.fi X-Virus-Scanned: amavisd-new at lists.oulu.fi From: "Sinikka Eskelinen" sinikka.eskelinen@oulu.fi To: ltkhkunta@lists.oulu.fi Date: Mon, 22 Oct 2007 14:21:36 +0300 X-Mailer: Microsoft Outlook Express 6.00.2900.3138 X-Mailman-Approved-At: Mon, 22 Oct 2007 14:31:31 +0300 Cc: Sinikka Eskelinen sinikka.eskelinen@oulu.fi Subject: [Ltkhkunta] Lectures in Modern Microscopy X-BeenThere: ltkhkunta@lists.oulu.fi X-Mailman-Version: 2.1.5 List-Id: ltkhkunta.lists.oulu.fi List-Unsubscribe: http://lists.oulu.fi/mailman/listinfo/ltkhkunta, mailto:ltkhkunta-request@lists.oulu.fi?subject=unsubscribe List-Archive: http://lists.oulu.fi/mailman/private/ltkhkunta List-Post: mailto:ltkhkunta@lists.oulu.fi List-Help: mailto:ltkhkunta-request@lists.oulu.fi?subject=help List-Subscribe: http://lists.oulu.fi/mailman/listinfo/ltkhkunta, mailto:ltkhkunta-request@lists.oulu.fi?subject=subscribe Sender: ltkhkunta-bounces@lists.oulu.fi

BIOCENTER <?xml:namespace prefix = st1 ns = "urn:schemas-microsoft-com:office:smarttags" />OULU laboratory course:<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" />

4th WORK SHOP ON

VISUALISATION OF CELLS AND TISSUES BY LIGHT AND ELECTRON MICROSCOPIC TECHNIQUES AND TOMOGRAPHIC METHODS

Place Auditorium F101 of the Department of Physiology and the Biocenter Oulu Laboratories of the Main Building of the Medical Campus, University of Oulu, Aapistie 5A, P.O.Box 5000, FIN-90014 Oulu, Finland

Time 24th to 26th October, 2007

Credits 1.5 ECTS units for lectures and demonstrations 1 to 4; 0.3 ECTS units for lectures only.

Organisers Biocenter Oulu Core Facilities at the BCO Tissue Imaging Center and the Departments of Medical Biochemistry and Molecular Biology and Pathology, University of Oulu, P.O. Box 5000, FIN-90014 University of Oulu, Finland

Aim To get acquainted with modern imaging technologies and collecting of images of living and fixed cultured cells as well as tissues using video microscope, laser scanning confocal microscope, electron microscope and optical tomography methods

Information Sinikka Eskelinen, Biocenter Oulu, Aapistie 5A, FIN-90014 University of Oulu, Finland, tel. 358-8-537 6101, fax 358-8-537 6115, e-mail mailto:sinikka.eskelinen@oulu.fisinikka.eskelinen@oulu.fi

URL: http://www.biocenter.oulu.fi/http://www.biocenter.oulu.fi/

Registration Registration for lectures recommended, registration for the demonstrations obligatory. Only 16 students are accepted for demonstrations. The application with a short description of the research project should be sent to Sinikka Eskelinen by September 28, 2007. Preference will be given to students whose projects best benefit the topics of the demonstrations given during the course.

PROGRAM

Wednesday, October 24th, 2007, Auditorium F101 of the Department of Physiology of the Medical Campus, Aapistie 7

Chair persons: Sinikka Eskelinen, Raija Sormunen, Aki Manninen

9.00 - 9.15 Opening words by Sinikka Eskelinen

9.15 - 10.15 Live-cell imaging with EosFP and other photoactivatable marker proteins of the GFP family

Jörg Wiedenmann, School of Ocean & Earth Science, University of Southampton National Oceanography Centre, Southampton European Way, Southampton, U.K.

10.15 - 11.15 Imaging Intracellular Signalling

Michael Courtney, Molecular Signalling Laboratory, Department of Neurobiology, A.I. Virtanen Institute, University of Kuopio, Finland

11.15 - 12.30 Lunch

12.30 - 13.30 Optical projection tomography: 3D microscopy of embryos and organs

Jamie Davies, University of Edinburgh, Edinburgh, Scotland, U.K.

13.30 - 14.30 Confocal microscopy of thick specimens

Nathalie Garin, Microscopy, Imaging & Morphology Facility, ISREC, Swiss Federal Institute of Technology in Lausanne, Switzerland

14.30 - 15.00 Coffee

15.00 - 16.00 Electron microscopic 3D imaging

Willie Geerts, Electron Microscopy Group Utrecht, University of Utrecth, The Netherlands

16:00 – 16.15 Concluding remarks by Raija Sormunen

Thursday and Friday, October 25th to 26th , 2007

Gathering in the meeting room 487B of Biocenter Oulu, Aapistie 5A, 4th floor, at 8.45 a.m. on Thursday and Friday morning for demonstrations.

Demonstration groups:

Group 1

1. Kirsi

Rilla Ph.D. Biomedicine/Kuopio

2. Ilkka Pietilä M.Sc. Med.biochem.

3. Fumi Suomi M.Sc. Biochemistry

4. Satu Myllymäki B.Sc. Med.biochem

5. Sanna Sillankorva M.Sc. Bioprocessing

Group 2

6. Katja Anttila M.Sc. Biology

7. Elina Kiiskilä B.Sc. Anatomy

8. Mari Aikio M.Sc. Med.biochem

9. Tuomas Huttunen Med.Cand. Med.biochem.

10. Henrika Honkanen M.Sc. Anatomy

Group 3

11. Antti Rivinoja M.Sc. Biochemistry

12. Anniina Vihervaara M.Sc. Turku, Biotech

13. Meng Wang Dipl.Eng Dept.Elect Eng

14. Timo Ruuska M.Sc. Surgery/CRC

15. Matti Kinnunen D.Sc.(Tech) Optoelectronics

Group 4

16. Antti Salo M.Sc. Biochemistry

17. Tiina Kantola M.Sc. Pathology

18. Anne Kunnari M.Sc. Internal med

19. Veli-Pekka Ronkainen M.Sc. Physiology

20. Marjut Tuominen B.Sc. Med biochem

Demonstrations 1 -4:

Thursday

Hours

1st group

2nd group

3rd group

4th group

9 - 12

Confocal microscopy 1

Confocal microscopy 2

Live cell imaging 1&2

Electron microscopy

12 - 13

lunch

lunch

lunch

lunch

13 - 16

Electron microscopy

Confocal microscopy 1

Confocal microscopy 2

Live cell imaging 1&2

Friday

Hours

1st group

2nd group

3rd group

4th group

9 - 12

Live cell imaging 1&2

Electron microscopy

Confocal microscopy 1

Confocal microscopy 2

12 - 13

lunch

lunch

lunch

lunch

13 - 16

Confocal microscopy 2

Live cell imaging 1&2

Electron microscopy

Confocal microscopy 1

Demonstrations:

1. Olympus confocal microscope 1: visualisation

of thick specimens, room 423A

Teacher: Nathalie Garin, Lauri Eklund, Mikko Nokkonen

2. Olympus confocal microscope 2 equipped with

second laser: photoactivatable marker proteins in living cells, room 422A

Teacher: Jörg Wiedenmann, Aki Manninen, Mikko Nokkonen

3. Live cell imaging 1 using Olympus inverted

microscope and Okolab chamber: long term cell culture and wound healing, room 421A

Teacher: Terhi Teräväinen and Riikka Wirkkala

Live cell imaging 2 using Olympus Cell M system: visualisation of cell organelles and cytoskeletal components in living cells, room 419A Teacher: Sinikka Eskelinen and Linda Rosberg

4. Electron microscopy: 3D tomography of EM

images, room 337B (microcomputer class)

Teacher: Willie Geerts, Raija Sormunen Sinikka Eskelinen, Ph.D. Coordinator Biocenter Oulu University of Oulu mailing address: P.O.Box 5000, FIN-90014 University of Oulu Finland street address: Aapistie 5A, FIN-90220 Oulu Tel. 358-8-5376101 Fax 358-8-5376115 e-mail mailto:sinikka.eskelinen@oulu.fisinikka.eskelinen@oulu.fi BIOCENTER http://www.biocenter.oulu.fiURL:http://www.biocenter.oulu.fi _______________________________________________ Ltkhkunta mailing list Ltkhkunta@lists.oulu.fi http://lists.oulu.fi/mailman/listinfo/ltkhkunta